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宿主来源
Rabbit抗原名称
α-tubulin分子别名
Tubulin alpha-4A chain; Alpha-tubulin 1免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
P68366抗体类型
Polyclonal antibody抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, HEK-293, NIH/3T3, mouse brain, C6, rat brain预测反应种属
(反应种属缩写表)Ar, Bv, Ck, Cz, Dr, Hm, Lob, Pg, Pl, Xe纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.
免疫印迹
WB result of α-tubulin Rabbit pAb
Primary antibody: α-tubulin Rabbit pAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDaWB result of α-tubulin Rabbit pAb
Primary antibody: α-tubulin Rabbit pAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDaWB result of α-tubulin Rabbit pAb
Primary antibody: α-tubulin Rabbit pAb at 1/5000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti- α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- α-tubulin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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