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宿主来源
Rabbit抗原名称
Granzyme A分子别名
CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor (H factor; HF); CTLA3; HFSP; GZMA免疫原
Recombinant Protein细胞定位
SecretedAccession
P12544抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
NK-92纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
Granzyme A (GzmA) is a serine protease predominantly found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It plays a critical role in immune defense by inducing cell death, both apoptotic and non-apoptotic, in target cells such as virus-infected or cancerous cells. Granzyme A is known to specifically target and cleave a range of important human proteins, including histones, nuclear envelope proteins, and key DNA repair proteins like Ku70 and PARP-1. Additionally, it can activate interleukin-1, contributing to pro-inflammatory activities. Recent research has highlighted Granzyme A's ability to trigger a novel programmed cell death pathway, pyroptosis, by cleaving Gasdermin B (GSDMB), which leads to the release of its N-terminal pore-forming fragment and subsequent cell death. This mechanism is particularly relevant in the context of anti-tumor immunity, as it suggests a new way in which cytotoxic lymphocytes can eliminate target cells. The discovery of Granzyme A's role in pyroptosis has provided new insights into the mechanisms of cell death induced by the immune system and offers potential therapeutic targets for enhancing anti-tumor immunity.
免疫印迹
WB result of Granzyme A Rabbit pAb
Primary antibody: Granzyme A Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NK-92 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
Observed MW: 22~29 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spleen. Anti-Granzyme A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human NK/T-cell lymphoma. Anti-Granzyme A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Granzyme A antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Granzyme A in tumor tissue.
Expression of Granzyme A in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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