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CDK1 Recombinant Rabbit mAb (S-1374-66)

Cyclin-dependent kinase 1,Cell division control protein 2 homolog,Cell division protein kinase 1,p34 protein kinase,CDC2,CDC28A,CDKN1,P34CDC2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1126
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产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CDK1

  • 分子别名

    Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDC2; CDC28A; CDKN1; P34CDC2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    P06493

  • 克隆号

    S-1374-66

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P ? ,
    ICC ? ,
    WB ,
    IP

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, Jurkat, MCF7, HT-29, NIH/3T3, mouse testis, C6, rat testis

  • 预测反应种属
    (反应种属缩写表)

    Bv, Ck, Or

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:2000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:200 Hu, Ms, Rt
ICC 1:500 Hu, Ms
背景介绍

  • CDK1, or Cyclin-dependent kinase 1, is a pivotal protein kinase that plays a critical role in controlling cell cycle progression. CDK1, in complex with cyclins (primarily cyclin B), is crucial for the progression of the cell cycle, particularly for the transition from G2 phase to mitosis (M phase). It helps in the initiation of mitosis by phosphorylating various substrates that are involved in chromosome condensation, nuclear envelope breakdown, and spindle assembly. CDK1 is involved in the regulation of transcription factors and chromatin modifiers, thereby influencing gene expression. It can modulate the activity of transcription factors like RUNX2, SIRT1/2, NPM1, and SREBF1, which mediate cell proliferation and apoptosis by modulating differentiation and metabolic processes. CDK1 plays a complex role in apoptosis. It can inhibit apoptosis through the phosphorylation of caspase-9 and BIRC5 proteins, but it can also promote cell death by phosphorylating and activating Bcl-2 family members. CDK1 regulates mitochondrial dynamics through the phosphorylation of proteins involved in mitochondrial fusion and fission. It also contributes to maintaining cellular redox balance and protects cells from oxidative stress.

  • 免疫印迹

    • WB result of CDK1 Recombinant Rabbit mAb
      Primary antibody: CDK1 Recombinant Rabbit mAb at 1/2000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: HT-29 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 34 kDa
      Observed MW: 30 kDa

    • WB result of CDK1 Recombinant Rabbit mAb
      Primary antibody: CDK1 Recombinant Rabbit mAb at 1/2000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 34 kDa
      Observed MW: 30 kDa

    • WB result of CDK1 Recombinant Rabbit mAb
      Primary antibody: CDK1 Recombinant Rabbit mAb at 1/2000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: rat testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 34 kDa
      Observed MW: 30 kDa

  • 免疫沉淀

    • CDK1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CDK1 in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using CDK1 Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: CDK1 Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 34 kDa
      Observed MW: 34 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-CDK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- CDK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti- CDK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of CDK1 in tumor tissue.

    • Expression of CDK1 in human tissue.

    • Expression of CDK1 in mouse & rat tissue.

  • 斯达特公司的抗体，可以回收利用几次？

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