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Rabbit抗原名称
GATA4分子别名
Transcription factor GATA-4; GATA-binding factor 4免疫原
Synthetic Peptide细胞定位
NucleusAccession
P43694克隆号
S-1428-141抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, mouse testis, rat testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Ms, Rat ICC 1:500 Hu IF 1:500 Ms, Rat
GATA4, or GATA binding protein 4, is a transcription factor that plays a crucial role in heart development, cardiomyocyte differentiation, and cardiac remodeling. It belongs to the GATA family of transcription factors, characterized by two zinc finger structures that enable DNA binding specificity. GATA4 is expressed in various cardiac cells, including myocardial cells, cardiac endothelial cells, and cardiac fibroblasts, and its mutation is closely associated with congenital heart diseases. Moreover, GATA4 is implicated in the regulation of gene expression related to cardiac hypertrophy and heart failure, where its activity can be modulated by various factors, including post-translational modifications such as acetylation and phosphorylation. It also interacts with other transcription factors like NKX2-5 and has been shown to be involved in the epigenetic regulation of cardiac-specific gene expression. The role of GATA4 extends beyond cardiac functions, as it is also implicated in the development and progression of various cancers, including breast cancer, where it regulates the expression of matrix metalloproteinase 9 (MMP9), affecting cell invasion and migration. The multifaceted functions of GATA4 underscore its significance in both cardiac biology and oncology.
免疫印迹
WB result of F GATA4 Recombinant Rabbit mAb
Primary antibody: GATA4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 45 kDa
Observed MW: 38~ 55 kDaWB result of F GATA4 Recombinant Rabbit mAb
Primary antibody: GATA4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse colon lysate 20 µg
Lane 2: mouse testis cell lysate 20 µg
Negative control: mouse colon lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 45 kDa
Observed MW: 38~55 kDaWB result of F GATA4 Recombinant Rabbit mAb
Primary antibody: GATA4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 45 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse testis. Anti-GATA4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-GATA4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HepG2 cells (top panel) and negative staining in HeLa cells (below panel). Anti-GATA4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse testis. Anti-GATA4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat testis. Anti-GATA4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of GATA4 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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