申请试用 NLRP3 Recombinant Rabbit mAb (S-1390-58)
NACHT,LRR and PYD domains-containing protein 3,Angiotensin/vasopressin receptor AII/AVP-like,Caterpiller protein 1.1 (CLR1.1),Cold-induced autoinflammatory syndrome 1 protein,Cryopyrin,PYRIN-containing APAF1-like protein 1,C1orf7,CIAS1,NALP3,PYPAF1
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宿主来源
Rabbit抗原名称
NLRP3分子别名
NACHT, LRR and PYD domains-containing protein 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1 (CLR1.1); Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1; C1orf7; CIAS1; NALP3; PYPAF1免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q96P20克隆号
S-1390-58抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
THP-1预测反应种属
(反应种属缩写表)M. mulatta纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
M13 bacteriophage is a filamentous virus that infects bacteria, particularly Escherichia coli (E. coli) strains showing the F-pilus. It is approximately 1 µm in length and 6 nm in width, consisting of a circular single-stranded DNA (ssDNA) molecule surrounded by a protein capsid. M13 has been extensively studied for its biological role and replication mechanism. Due to advancements in molecular biology techniques, M13 has become an intriguing nanotool for various applications. One of its notable capabilities is 'phage display,' which allows M13 to display fused peptides and proteins on its minor and major coat proteins. This has paved the way for studying protein-protein and peptide-protein interactions, developing molecular probes for cell imaging, and detecting microorganisms.
免疫印迹
WB result of NLRP3 Recombinant Rabbit mAb
Primary antibody: NLRP3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: untreated THP-1 whole cell lysate 20 µg
Lane 3: THP-1 treated with 80nM TPA for 16 hours and 1ug/ml LPS for 1h whole cell lysate 20 µg
Weak expression: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 118 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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