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宿主来源
Rabbit抗原名称
NLRP3分子别名
NACHT, LRR and PYD domains-containing protein 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1 (CLR1.1); Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1; C1orf7; CIAS1; NALP3; PYPAF1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q96P20克隆号
S-1390-58抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Hu阳性样本
THP-1预测反应种属
(反应种属缩写表)M. mulatta纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
NLRP3, or NOD-like receptor protein 3, is a key component of the inflammasome complex that plays a critical role in the innate immune system. NLRP3 forms a complex with the adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD) and pro-caspase-1. This complex, known as the NLRP3 inflammasome, is crucial for the activation of caspase-1, which then processes IL-1β and IL-18 into their mature forms. NLRP3 inflammasome activation also leads to a form of programmed cell death known as pyroptosis, characterized by cell membrane rupture and the release of pro-inflammatory intracellular contents. Conditions such as gout, rheumatoid arthritis, and multiple sclerosis are associated with NLRP3 inflammasome activation. Furthermore, in Alzheimer's disease and Parkinson's disease, NLRP3 inflammasome activation contributes to neuroinflammation and neuronal damage.
免疫印迹
WB result of NLRP3 Recombinant Rabbit mAb
Primary antibody: NLRP3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: untreated THP-1 whole cell lysate 20 µg
Lane 3: THP-1 treated with 80nM TPA for 16 hours and 1ug/ml LPS for 1h whole cell lysate 20 µg
Weak expression: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 118 kDa
This blot was developed with high sensitivity substrate
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