Caspase-7 Recombinant Rabbit mAb (S-782-50)

CASP-7,Apoptotic protease Mch-3,Cysteine protease LICE2,Lice2,Mch3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1117

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Caspase-7
分子别名

CASP-7; Apoptotic protease Mch-3; Cysteine protease LICE2; Lice2; Mch3
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus, Secreted
Accession

P97864
克隆号

S-782-50
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
阳性样本

RAW264.7, C2C12, NIH/3T3, mouse heart, PC-12, C6
预测反应种属
(反应种属缩写表)

Ck, Pg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IP 1:50 Ms
IHC-P 1:500 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IP	1:50	Ms
IHC-P	1:500	Ms, Rt

背景介绍

Caspase-7 is a member of the caspase family of proteins, which are cysteine proteases playing a central role in the execution of apoptosis, or programmed cell death. Caspase-7, along with Caspase-3 and Caspase-6, is classified as an executioner caspase. These caspases are responsible for the demolition phase of apoptosis by directly degrading cellular structural and functional proteins, leading to the characteristic features of cell death. Caspase-7 is activated by initiator caspases such as Caspase-8 and Caspase-9 during death receptor and DNA damage-induced apoptosis, respectively. It is processed from its inactive zymogen form to its active form, which then cleaves a set of cellular substrates. Recent research indicates that Caspase-7 also plays a role in inflammation. Caspase-7 deficient mice are resistant to endotoxemia, suggesting that interfering with Caspase-7 activation may have therapeutic value for the treatment of inflammatory diseases. A novel function of Caspase-7 has been discovered in the repair of plasma membrane pores. Caspase-7 facilitates the repair of Gasdermin D and perforin pores by activating acid sphingomyelinase (ASM), which generates ceramide for membrane repair. This function is independent of Caspase-3 activity and is crucial for maintaining cell membrane integrity during inflammatory and apoptotic processes. Moreover, Caspase-7 has been implicated in the defense against intracellular pathogens. It can be activated by pyroptotic Caspase-1 in intestinal epithelial cells or act downstream of granzyme B in cells under attack by natural killer (NK) cells or cytotoxic T lymphocytes (CTLs).

免疫印迹
- WB result of Caspase-7 Recombinant Rabbit mAb
  Primary antibody: Caspase-7 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: RAW264.7 whole cell lysate 20 µg
  Lane 2: C2C12 whole cell lysate 20 µg
  Lane 3: NIH/3T3 whole cell lysate 20 µg
  Lane 4: mouse heart lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 34 kDa
  Observed MW: 32, 37 kDa
- WB result of Caspase-7 Recombinant Rabbit mAb
  Primary antibody: Caspase-7 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 34 kDa
  Observed MW: 32, 37 kDa
免疫沉淀
- Caspase 7 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Caspase 7 in 0.4 mg RAW264.7 whole cell lysate.
  Western blot was performed on the immunoprecipitate using Caspase 7 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: RAW264.7 whole cell lysate 20 µg (Input)
  Lane 2: Caspase 7 Rabbit mAb IP in RAW264.7 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in RAW264.7 whole cell lysate
  Predicted MW: 34 kDa
  Observed MW: 32, 37 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse stomach. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of Caspase-7 in mouse & rat tissue.