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ENPP3/CD203c Recombinant Rabbit mAb (S-1632-11)

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3,E-NPP 3,NPP3,Alkaline phosphodiesterase I,Dinucleoside polyphosphatase,Nucleotide diphosphatase,Nucleotide pyrophosphatase (NPPase),Phosphodiesterase I beta (PD-Ibeta),Phosphodiesterase I/nucleotide pyrophosphatase 3,PDNP3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1115
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ENPP3/CD203c

  • 分子别名

    Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; E-NPP 3; NPP3; Alkaline phosphodiesterase I; Dinucleoside polyphosphatase; Nucleotide diphosphatase; Nucleotide pyrophosphatase (NPPase); Phosphodiesterase I beta (PD-Ibeta); Phosphodiesterase I/nucleotide pyrophosphatase 3; PDNP3

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted, Cell membrane

  • Accession

    O14638

  • 克隆号

    S-1632-11

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    KU-812, Neruo-2a, mouse kidney

  • 预测反应种属
    (反应种属缩写表)

    P. abelii

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:250 Hu, Ms
    IF 1:500 Hu
背景介绍

  • ENPP3 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 3) is a type II transmembrane protein belonging to the membrane-bound ectonucleotide pyrophosphatase/phosphodiesterase family (ENPPs/ENPPs). ENPP3 is involved in nucleotide signaling, particularly in regulating allergic responses in basal cells and mast cells, and serves as a potential biomarker for allergen sensitivity. Under pathological conditions, ENPP3 is overexpressed in various cancers, including renal cell carcinoma, hepatocellular carcinoma, colorectal cancer, and cholangiocarcinoma, making it a potential tumor marker and therapeutic target. ENPP3 is recognized as a major cGAMP hydrolase, which is a second messenger synthesized after the detection of cytosolic double-stranded DNA and is transmitted intercellularly to promote downstream immune signaling. Like ENPP1, ENPP3 inhibits anti-tumor immune responses by hydrolyzing cGAMP.

  • 免疫印迹

    • WB result of ENPP3/CD203c Recombinant Rabbit mAb
      Primary antibody: ENPP3/CD203c Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Daudi whole cell lysate 20 µg
      Lane 2: KU-812 whole cell lysate 20 µg
      Negative control: Daudi whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 100 kDa
      Observed MW: 150 kDa

    • WB result of ENPP3/CD203c Recombinant Rabbit mAb
      Primary antibody: ENPP3/CD203c Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 100 kDa
      Observed MW: 140 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ENPP3/CD203c antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human endometrial cancer. Anti- ENPP3/CD203c antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of ENPP3/CD203c in tumor tissue.

    • Expression of ENPP3/CD203c in human tissue.

    • Expression of ENPP3/CD203c in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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