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GSK3β Recombinant Rabbit mAb (S-1209-170)

Glycogen synthase kinase-3 beta,GSK-3 beta,Serine/threonine-protein kinase GSK3B

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1114
规格
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    GSK3β

  • 分子别名

    Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus, Cell membrane

  • Accession

    P49841

  • 克隆号

    S-1209-170

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, A431, MCF7, NIH/3T3, PC-12

  • 预测反应种属
    (反应种属缩写表)

    S. citellus

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IP 1:50 Hu
    IHC-P 1:500 Hu, Ms, Rt
    ICC 1:500 Hu, Ms
背景介绍

  • GSK-3β (Glycogen Synthase Kinase 3 beta) is a multifunctional serine/threonine protein kinase that is highly conserved across eukaryotes. GSK-3β is expressed in all cell types and is particularly abundant in the brain. It regulates cell proliferation, survival, gene expression, cellular architecture, and neural development and plasticity. Dysregulation of GSK-3β is implicated in numerous disorders, including cancers, diabetes, and neurodegenerative diseases. In Alzheimer’s disease, GSK-3β contributes to the pathological phosphorylation of tau protein. GSK-3β plays a significant role in neuronal apoptosis, and its inhibition reduces the expression of alpha-Synuclein (α-Synuclein), making it a potential therapeutic target for neurodegenerative disorders like Parkinson's disease (PD).

  • 免疫印迹

    • WB result of GSK3β Recombinant Rabbit mAb
      Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: A431 whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 46 kDa

    • WB result of GSK3β Recombinant Rabbit mAb
      Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 46 kDa

    • WB result of GSK3β Recombinant Rabbit mAb
      Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48 kDa
      Observed MW: 46 kDa

  • 免疫沉淀

    • GSK3β Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating GSK3β in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using GSK3β Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: GSK3β Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 48 kDa
      Observed MW: 46 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- GSK3β antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti- GSK3β antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of GSK3β in tumor tissue.

    • Expression of GSK3β in human tissue.

    • Expression of GSK3β in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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