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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H2B (mono methyl K5) Recombinant Rabbit mAb (S-1416-412)

Histone H2B type 2-E,H2B-clustered histone 21,Histone H2B-GL105,Histone H2B.q (H2B/q),H2BC21,H2BFQ,HIST2H2BE

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1113
规格
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H2B (mono methyl K5)

  • 分子别名

    Histone H2B type 2-E; H2B-clustered histone 21; Histone H2B-GL105; Histone H2B.q (H2B/q); H2BC21; H2BFQ; HIST2H2BE

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q16778

  • 克隆号

    S-1416-412

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    甲基化

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, MCF7, LNCaP, NIH/3T3, C6

  • 预测反应种属
    (反应种属缩写表)

    Zf, Ck, Or, Cw, X. laevis, M. fascicularis, C. moschata, C. niloticus

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ChIP

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
    ICC 1:500 Hu, Ms
    ChIP 1:20-1:50 Hu
背景介绍

  • Histone H2B (mono methyl K5) is a specific modification of the histone protein H2B at lysine residue 5, where a single methyl group is added. Histone H2B (mono methyl K5) is a core component of the nucleosome, which is essential for the packaging of DNA into chromatin. This modification, along with others, contributes to the regulation of DNA accessibility, thereby influencing transcription regulation, DNA repair, DNA replication, and chromosomal stability. Histone H2B mono methylation, particularly at K5, has been implicated in transcriptional regulation. It is part of a complex set of post-translational modifications known as the "histone code," which affects gene expression.

  • 免疫印迹

    • WB result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
      Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: LNCaP whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 14 kDa
      Observed MW: 17 kDa
      This blot was developed with high sensitivity substrate

    • WB result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
      Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 14 kDa
      Observed MW: 17 kDa
      This blot was developed with high sensitivity substrate

    • WB result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
      Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 14 kDa
      Observed MW: 17 kDa
      This blot was developed with high sensitivity substrate

  • 斑点杂交

    • Dot blot result of Histone H2B (mono methyl K5) Recombinant Rabbit mAb
      Lane 1: Histone H2B (mono methyl K5) peptide
      Lane 2: Histone H2B unmodified peptide
      Lane 3: Histone H2B (di methyl K5) peptide
      Lane 4: Histone H2B (tri methyl K5) peptide
      Primary antibody: Histone H2B (mono methyl K5) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-Histone H2B (mono methyl K5) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Histone H2B (mono methyl K5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti- Histone H2B (mono methyl K5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used H2B mono methyl K5 Recombinant Rabbit mAb (S-1416-412) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA)
      showed the enrichment of RPL30, GAPDH, MYOD1,
      AFM, SAT-α and SAT-2 in H2B mono methyl K5 Recombinant
      Rabbit mAb (S-1416-412)-immunoprecipitated sample.

  • 组织表达图谱

    • Expression of Histone H2B (mono methyl K5) in tumor tissue.

    • Expression of Histone H2B (mono methyl K5) in human tissue.

    • Expression of Histone H2B (mono methyl K5) in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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