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Rabbit抗原名称
hnRNP H分子别名
Heterogeneous nuclear ribonucleoprotein H; HNRNPH1; HNRPH; HNRPH1免疫原
Synthetic Peptide细胞定位
NucleusAccession
P31943抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Molt-4, HeLa, 293T, U-87 MG, Jurkat, NIH/3T3, mouse brain, C6, rat brain纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:1000 Hu, Ms
Heterogeneous nuclear ribonucleoprotein H (hnRNP H) is a member of the hnRNP family, which plays crucial roles in RNA metabolism, including alternative splicing, mRNA stabilization, and transcriptional and translational regulation. hnRNP H is involved in different stages of mRNA metabolism, including binding to promotor and enhancer sequences to direct transcription, stabilizing newly formed transcripts, regulating alternative splicing, and controlling translation. The expression level of hnRNP H is altered in many types of cancer, suggesting its role in tumorigenesis. Additionally, hnRNP H has been linked to various neurodegenerative diseases, such as spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), Alzheimer’s disease (AD), and fronto-temporal lobe dementia (FTLD). hnRNP H has been shown to associate with hTERC (human telomerase RNA component) and modulate telomerase function, which is important for maintaining telomere length and has implications in cancer and stem cell biology.
免疫印迹
WB result of hnRNP H Rabbit pAb
Primary antibody: hnRNP H Rabbit pAb at 1/1000 dilution
Lane 1: Molt-4 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50 kDaWB result of hnRNP H Rabbit pAb
Primary antibody: hnRNP H Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50 kDaWB result of hnRNP H Rabbit pAb
Primary antibody: hnRNP H Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-hnRNP H antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-hnRNP H antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-hnRNP H antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-hnRNP H antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-hnRNP H antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-hnRNP H antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-hnRNP H antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-hnRNP H antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-hnRNP H antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of hnRNP in tumor tissue.
Expression of hnRNP in human tissue.
Expression of hnRNP in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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