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Rabbit抗原名称
Lamin A分子别名
LMNA免疫原
Synthetic Peptide细胞定位
Nucleus laminaAccession
P02545克隆号
S-1503-44抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB, IP反应种属 ?
Hu阳性样本
A431, HeLa, PANC-1, MCF7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:10000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Lamin A is a type V intermediate filament protein essential for maintaining the integrity and mechanical properties of the nucleus. It is part of the nuclear lamina, which is a network of proteins beneath the inner nuclear membrane that helps determine the shape and size of the nucleus. Lamin A is involved in the regulation of gene expression and cell cycle progression. It interacts with the nuclear envelope and chromatin, influencing cellular processes such as DNA replication, cell cycle regulation, and signal transduction. Lamin A also serves as a "mechanosensor," linking the cytoskeleton and nuclear structures, and plays a role in the response to mechanical stress within the cell. Overexpression of Lamin A/C protein has been observed in invasive prostate cancer and is implicated in promoting cancer cell growth, migration, and invasion through the PI3K/AKT/PTEN pathway.
免疫印迹
WB result of Lamin A Recombinant Rabbit mAb
Primary antibody: Lamin A Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 74 kDa
Observed MW: 68 kDa
免疫沉淀
Lamin A Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Lamin A in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Lamin A Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: Lamin A Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 74 kDa
Observed MW: 74 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Lamin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Lamin A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Lamin A in tumor tissue.
Expression of Lamin A in human tissue.
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