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Rabbit抗原名称
Syntenin分子别名
Melanoma differentiation-associated protein 9 (MDA-9); Pro-TGF-alpha cytoplasmic domain-interacting protein 18 (TACIP18); Scaffold protein Pbp1; Syndecan-binding protein 1; SDCBP; MDA9; SYCL免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
O00560克隆号
S-1692-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, A549, HepG2纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IP 1:50 Hu IHC-P 1:250 Hu ICC 1:500 Hu
Syntenin is a multifunctional intracellular adaptor protein that plays a crucial role in various signaling pathways and cellular functions. It is composed of two adjacent tandem PDZ (PSD-95/Discs large/Zonula occludens-1) domains, which recognize multiple peptide motifs with low to moderate affinity. Syntenin interacts with a plethora of proteins, regulating the architecture of the cell membrane. Increases in Syntenin levels can induce the metastasis of tumor cells, neurite protrusion in neuronal cells, and exosome biogenesis in various cell types. It is also involved in the subcellular trafficking of binding partners during endocytic and exocytic events. Additionally, syntenin interacts with the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2), which influences its localization and function at the plasma membrane. Furthermore, syntenin is involved in the regulation of exosome biogenesis, which is a key process in intercellular communication, including neuron-neuron, neuron-glia, and glia-glia crosstalk, as well as in tumor metastasis, cell proliferation, and angiogenesis. Syntenin interacts with proteins involved in exosome biogenesis, indicating its role in intracellular membrane rearrangement and cargo loading into exosomes.
免疫印迹
WB result of Syntenin Recombinant Rabbit mAb
Primary antibody: Syntenin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 32 kDa
免疫沉淀
Syntenin Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Syntenin in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Syntenin Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: Syntenin Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 32 kDa
Observed MW: 34 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Syntenin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Syntenin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Syntenin in tumor tissue.
Expression of Syntenin in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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