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nNOS Recombinant Rabbit mAb (S-1641-70)

Nitric oxide synthase 1,Constitutive NOS,NC-NOS,NOS type I,Neuronal NOS (N-NOS,nNOS),Nitric oxide synthase,brain (bNOS),Peptidyl-cysteine S-nitrosylase NOS1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1106

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

nNOS
分子别名

Nitric oxide synthase 1; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS (N-NOS; nNOS); Nitric oxide synthase, brain (bNOS); Peptidyl-cysteine S-nitrosylase NOS1
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P29475
克隆号

S-1641-70
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
预测反应种属
(反应种属缩写表)

Hu, Sh, Rb
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IHC-P 1:200 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IHC-P	1:200	Ms, Rt

背景介绍

Neuronally-derived Nitric Oxide Synthase (nNOS or NOS1) is a type of nitric oxide synthase enzyme. nNOS is mainly found in neurons and plays a crucial role in the central nervous system, particularly in synaptic signaling and plasticity. It is also present in skeletal muscle, where it regulates muscle contractility and local blood flow. nNOS interacts with a number of proteins, including post-synaptic density protein 95 (PSD-95), which is crucial for its localization at the postsynaptic density near the N-methyl-D-aspartate receptor (NMDAR). This interaction is important for coupling calcium influx through NMDA receptors to NO synthesis. nNOS has been implicated in various neuropathological states, including cognitive and motor deficits associated with early synaptic loss. It also negatively regulates neurogenesis under physiological and pathological conditions.

免疫印迹
- WB result of nNOS Recombinant Rabbit mAb
  Primary antibody: nNOS Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse liver lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Negative control: mouse liver lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 161 kDa
  Observed MW: 160 kDa
- WB result of nNOS Recombinant Rabbit mAb
  Primary antibody: nNOS Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat liver lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Negative control: rat liver lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 161 kDa
  Observed MW: 160 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse colon. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat colon. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of nNOS in mouse & rat tissue.