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Rabbit抗原名称
Osteopontin分子别名
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1 (SPP-1); Urinary stone protein; Uropontin; SPP1; BNSP; OPN免疫原
Recombinant Protein细胞定位
SecretedAccession
P10451克隆号
S-1005-50抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IP 1:50 Hu IHC-P 1:1000 Hu
Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a multifunctional protein that plays significant roles in various biological processes. OPN is associated with numerous inflammatory conditions, including infections, allergic responses, autoimmunity, and tissue damage. It is expressed by many cell types in the peripheral immune system and has various functions that can be either beneficial or detrimental. OPN is involved in bone metabolism and homeostasis, playing a role in neuron-mediated and endocrine-regulated bone mass. It is secreted by osteoblasts and other cells in the bone marrow and is important for the proliferation, migration, and adhesion of bone-related cells such as bone marrow mesenchymal stem cells, hematopoietic stem cells, osteoclasts, and osteoblasts. It has been implicated in the occurrence and development of several bone-related diseases, such as osteoporosis, rheumatoid arthritis, and osteosarcoma. It is also linked to cardiovascular diseases, cancer, diabetes, and kidney stone diseases. Moreover, OPN promotes tumor cell growth and induces anti-apoptotic signaling through its activation of CD44 and integrin heterodimers. It enhances tumor cell mobility, invasiveness, and epithelial-mesenchymal transition, which are crucial for dissemination and metastasis.
免疫印迹
WB result of Osteopontin Recombinant Rabbit mAb
Primary antibody: Osteopontin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 70 kDa
免疫沉淀
Osteopontin Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Osteopontin in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using Osteopontin Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: Osteopontin Recombinant Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 35 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Osteopontin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Osteopontin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Osteopontin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Osteopontin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Osteopontin in tumor tissue.
Expression of Osteopontin in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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