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Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb (S-985-212)

Ribosomal protein S6 kinase alpha-1,S6K-alpha-1,90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1,p90RSK1,p90S6K),MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a,MAPKAP kinase 1a,MAPKAPK-1a),Ribosomal S6 kinase 1 (RSK-1),RPS6KA1,MAPKAPK1A,RSK1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1103

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-p90 RSK (T359/S363)
分子别名

Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1; p90RSK1; p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); RPS6KA1; MAPKAPK1A; RSK1
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

Q15418
克隆号

S-985-212
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
应用

WB
反应种属 ?

Hu, Ms, Rt
阳性样本

HepG2, HeLa
预测反应种属
(反应种属缩写表)

Ck, Xe
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度	推荐种属
WB	1:1000	Hu,Ms,Rt

背景介绍

p90 RSK, also known as RSK1, is a member of the 90 kDa ribosomal S6 kinase family, which includes RSK1, RSK2, and RSK3. These are serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2. RSK1 is activated by MAPK in vitro and in vivo via phosphorylation. Active RSKs play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB, which are key regulators of gene expression. The phosphorylation of RSK1 at T359/S363 is part of the signaling cascade initiated by growth factors and hormones, which leads to cellular proliferation and survival.

免疫印迹
- WB result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
  Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HepG2 whole cell lysate 20 µg
  Lane 2: HepG2 treated with 100 ng/ml EGF for 20 minutes whole cell lysate 20 µg
  Lane 3: untreated HeLa whole cell lysate 20 µg
  Lane 4: HeLa treated with 60 µg/ml TPA for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 83 kDa
  Observed MW: 90 kDa
- WB result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
  Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HepG2 treated with 100 ng/ml EGF for 20 minutes whole cell lysate 20 µg
  Lane 2: HeLa treated with 60 µg/ml TPA for 30 minutes whole cell lysate 20 µg
  Lane 3: HepG2 treated with 100 ng/ml EGF for 20 minutes, then treated with phosphatase whole cell lysate 20 µg
  Lane 4: HeLa treated with 60 µg/ml TPA for 30 minutes, then treated with phosphatase whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 83 kDa
  Observed MW: 90 kDa
斑点杂交
- Dot blot result of Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb
  Lane 1: p90 RSK (T359/S363) phospho peptide
  Lane 2: p90 RSK (S363) phospho peptide
  Lane 3: p90 RSK (T359) phospho peptide
  Lane 4: p90 RSK unmodified peptide
  Primary antibody: Phospho-p90 RSK (T359/S363) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution