分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
PSD-95分子别名
Disks large homolog 4; Postsynaptic density protein 95; Synapse-associated protein 90 (SAP-90; SAP90); Dlg4; Dlgh4；PSD95免疫原
Recombinant Protein细胞定位
Cytoplasm, Cell membraneAccession
Q62108克隆号
S-1518-4抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse cerebellum, mouse brain, rat cerebellum, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:500 Hu, Ms, Rt IF 1:500 Hu
PSD-95 (Postsynaptic Density Protein-95) is a protein highly enriched in the postsynaptic density of excitatory synapses in the central nervous system. It is a major synaptic scaffolding protein that contains three PDZ domains, one SH3 domain, and a GUK domain. These domains enable PSD-95 to interact with a variety of proteins, including NMDA and AMPA receptors, thereby playing a crucial role in synaptic plasticity and signal transduction. PSD-95 is involved in regulating the maturation and function of synapses, influencing long-term potentiation (LTP) and long-term depression (LTD). It modulates the stability and function of receptors and ion channels at the postsynaptic membrane through interactions with them. Abnormalities in PSD-95 function are associated with various neuropsychiatric disorders, including autism, schizophrenia, and intellectual disability. Mutations in the gene encoding PSD-95 in these disorders may lead to synaptic dysfunction.
免疫印迹
WB result of PSD-95 Recombinant Rabbit mAb
Primary antibody: PSD-95 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 80 kDa
Observed MW: 70~110 kDaWB result of PSD-95 Recombinant Rabbit mAb
Primary antibody: PSD-95 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 81 kDa
Observed MW: 70~110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human retina. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human cerebellum. Anti- PSD-95 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of PSD-95 in human tissue.
Expression of PSD-95 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)