APOBEC3B Rabbit Polyclonal Antibody

DNA dC->dU-editing enzyme APOBEC-3B,A3B,Phorbolin-1-related protein,Phorbolin-2/3

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B1100

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

APOBEC3B
分子别名

DNA dC->dU-editing enzyme APOBEC-3B; A3B; Phorbolin-1-related protein; Phorbolin-2/3
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

Q9UH17
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

A431, HepG2, HeLa, HCT 116, HaCaT, HCC827, U-2 OS
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
ICC	1:500	Hu

背景介绍

APOBEC3B (Apolipoprotein B mRNA editing enzyme catalytic subunit 3B) is a member of the human APOBEC (Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like) family, which includes enzymes that deaminate cytosine (C) to uracil (U) in RNA and single-stranded DNA. APOBEC3B is a DNA cytosine deaminase that can convert cytosine in DNA to uracil. This conversion, if not repaired, can lead to C to T transition mutations during DNA replication. Its role in tumorigenesis in cancer development is achieved by promoting mutation processes. These mutations provide genetic diversity for cancer, thereby promoting cancer invasion, metastasis, recurrence, and therapeutic resistance.

免疫印迹
- WB result of APOBEC3B Rabbit pAb
  Primary antibody: APOBEC3B Rabbit pAb at 1/1000 dilution
  Lane 1: SK-BR-3 whole cell lysate 20 µg
  Lane 2: A431 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: HeLa whole cell lysate 20 µg
  Lane 5: HCT 116 whole cell lysate 20 µg
  Lane 6: HaCaT whole cell lysate 20 µg
  Lane 7: HCC827 whole cell lysate 20 µg
  Lane 8: U-2 OS whole cell lysate 20 µg
  Negative control: SK-BR-3 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 46 kDa
  Observed MW: 35 kDa
流式分析
免疫细胞化学
- ICC shows positive staining in A431 cells. Anti-APOBEC3B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).