大包装询价 产品介绍 引用文献(0) 评论(0)
宿主来源
Rabbit抗原名称
ROCK2分子别名
Rho-associated protein kinase 2; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II (ROCK-II); p164 ROCK-2; KIAA0619免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, NucleusAccession
O75116抗体类型
Polyclonal antibody抗体同种型
IgG应用
IHC-P, ICC, WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, HEK-293, MCF7, NIH/3T3, C2C12, mouse brain, PC-12, rat brain纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
ROCK2 (Rho-associated kinase 2) is a serine/threonine protein kinase that belongs to the Rho GTPase effector family and plays a crucial role in the regulation of the cytoskeleton dynamics, cell migration, proliferation, and survival. ROCK2 has been identified as a key driver in the development of various cancers. In acute myeloid leukemia (AML), increased expression of ROCK2 leads to lower rates of complete remission and poorer overall survival. Inhibition of ROCK2 can enhance the sensitivity of AML cell lines and primary AML samples to drugs and reduce the activity of downstream signaling pathways. ROCK2 is a key modulator in immune regulation and fibrosis and has become a therapeutic target for chronic graft-versus-host disease (cGVHD). ROCK2 plays a primary regulatory role in axonal degeneration, neuronal death, and axonal regeneration in the central nervous system (CNS). Downregulation of ROCK2 can inhibit apoptosis, reduce axonal degeneration, and promote neurite outgrowth.
免疫印迹
WB result of ROCK2 Rabbit pAb
Primary antibody: ROCK2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 161 kDa
Observed MW: 160 kDaWB result of ROCK2 Rabbit pAb
Primary antibody: ROCK2 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse brain whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 161 kDa
Observed MW: 160 kDaWB result of ROCK2 Rabbit pAb
Primary antibody: ROCK2 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 161 kDa
Observed MW: 160 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-ROCK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ROCK2 in tumor tissue
Expression of ROCK2 in human tissue
Expression of ROCK2 in mouse & rat tissue
引用文献(0)
评论(0)