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Peroxiredoxin 6 Rabbit Polyclonal Antibody

1-Cys peroxiredoxin (1-Cys PRX),24 kDa protein,Acidic calcium-independent phospholipase A2 (aiPLA2),Antioxidant protein 2,Glutathione-dependent peroxiredoxin,Liver 2D page spot 40,Lysophosphatidylcholine acyltransferase 5 (LPC acyltransferase 5,LPCAT-5,Lyso-PC acyltransferase 5),Non-selenium glutathione peroxidase (NSGPx),Red blood cells page spot 12,PRDX6,AOP2,KIAA0106

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1098
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Peroxiredoxin 6

  • 分子别名

    1-Cys peroxiredoxin (1-Cys PRX); 24 kDa protein; Acidic calcium-independent phospholipase A2 (aiPLA2); Antioxidant protein 2; Glutathione-dependent peroxiredoxin; Liver 2D page spot 40; Lysophosphatidylcholine acyltransferase 5 (LPC acyltransferase 5; LPCAT-5; Lyso-PC acyltransferase 5); Non-selenium glutathione peroxidase (NSGPx); Red blood cells page spot 12; PRDX6; AOP2; KIAA0106

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Lysosome, Cytoplasm

  • Accession

    P30041

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    A431, HeLa, HEK-293, MCF7, PC-3, mouse liver, mouse testis, rat liver, rat testis

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IP 1:50 Hu
    IHC-P 1:200 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • Peroxiredoxin-6 (Prdx6) is a unique member of the peroxiredoxin family of antioxidant enzymes. Unlike other peroxiredoxins, Prdx6 contains only one conserved cysteine residue and exhibits three types of activities: peroxidase activity, phospholipase A2 (PLA2) activity, and lysolecithin acyltransferase (LPCAT) activity. These activities are crucial for antioxidant defense, cellular phospholipid balance, and the generation of superoxide anion via activation of NADPH oxidase signaling cascades. The regulation of Prdx6 activities is influenced by subcellular localization, substrate binding, and post-translational modifications. Prdx6 plays a significant role in the reduction of peroxidized membrane phospholipids and in the phospholipid homeostasis by generating lysophospholipid substrates for the remodeling pathway of phospholipid synthesis. Prdx6 is closely associated with the pathogenesis of various diseases, including cancer, inflammatory diseases, ischemic stroke, traumatic brain injury, and neurodegenerative diseases. Moreover, Prdx6 has been implicated in the repair of peroxidized cell membranes and cell signaling, highlighting its importance in maintaining cellular integrity and function. The enzyme's ability to reduce oxidized sn-2 fatty acyl groups of phospholipids and to hydrolyze the sn-2 ester bond of phospholipids contributes to its bifunctional role in antioxidant defense and phospholipid homeostasis.

  • 免疫印迹

    • WB result of Peroxiredoxin 6 Rabbit pAb
      Primary antibody: Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution
      Lane 1: A431 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: HEK-293 whole cell lysate 20 µg
      Lane 4: MCF7 whole cell lysate 20 µg
      Lane 5: PC-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 25 kDa
      Observed MW: 28 kDa

    • WB result of Peroxiredoxin 6 Rabbit pAb
      Primary antibody: Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution
      Lane 1: mouse liver lysate 20 µg
      Lane 2: mouse testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 25 kDa
      Observed MW: 27 kDa

    • WB result of Peroxiredoxin 6 Rabbit pAb
      Primary antibody: Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution
      Lane 1: rat liver lysate 20 µg
      Lane 2: rat testis lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 25 kDa
      Observed MW: 26 kDa

  • 免疫沉淀

    • Peroxiredoxin 6 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating Peroxiredoxin 6 in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using Peroxiredoxin 6 Rabbit pAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: Peroxiredoxin 6 Rabbit pAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 25 kDa
      Observed MW: 26 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-Peroxiredoxin 6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Peroxiredoxin 6 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Peroxiredoxin 6 in tumor tissue

    • Expression of Peroxiredoxin 6 in human tissue

    • Expression of Peroxiredoxin 6 in mouse & rat tissue

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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