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Rabbit抗原名称
RFP分子别名
Red fluorescent protein drFP583, DsRed免疫原
Recombinant ProteinAccession
Q9U6Y8克隆号
S-1006-109抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Species Independent交叉反应
This antibody is reactive against variants of RFP such as mCherry, mRFP, mPlum, mOrange, PA-mCherry and mScarlet
纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Species Independent ICC 1:500 Species Independent
Red fluorescent protein, also known as RFP, is a type of protein that emits red fluorescence under ultraviolet light. It is an essential optical imaging tool in the field of life science. RFP can be used for live cell imaging, marking protein expression, and various protein-related experiments. It is one of the most commonly used fluorescent proteins, along with green fluorescent protein (GFP) and orange fluorescent protein (OFP). RFP has several advantages compared to GFP. Firstly, RFP can be used in combination with GFP to address scientific issues that GFP alone cannot solve. Secondly, RFP has longer excitation and emission wavelengths, which results in a lower background during intracellular imaging.
免疫印迹
WB result of RFP Recombinant Rabbit mAb
Primary antibody: RFP Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T transfected with empty vector whole cell Lysate 20 µg
Lane 3: 293T transfected with Histone H3-RFP-Avi Tag whole cell Lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 48 kDaWB result of RFP Recombinant Rabbit mAb
Primary antibody: RFP Rabbit mAb at 1/10000 dilution
Lane 1: RFP protein 20 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 28 kDa
免疫细胞化学
ICC shows negative staining in vector-transfected 293T cells. Anti-RFP antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in Histone H3-RFP-Avi Tag transfected 293T cells. Anti-RFP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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