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宿主来源
Rat抗原名称
CD8α分子别名
Lyt-2细胞定位
Cell membraneAccession
P01731克隆号
53-6.7抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1/20000 Ms
CD8α encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD8α antibody at 1/20000 (0.001 μg) dilution (Right) compared with a Rat monoclonal IgG isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD4 - Alexa Fluor® 647 separately. Gated on total viable cells.
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