Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-515-360)

Signal transducer and activator of transcription 3,Acute-phase response factor,APRF

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1094

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Stat3 (Tyr705)
分子别名

Signal transducer and activator of transcription 3; Acute-phase response factor; APRF
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P40763
克隆号

S-515-360
抗体类型

Recombinant mAb
抗体同种型

IgG
应用

ChIP ,
ICC ? ,
WB ,
IP
反应种属 ?

Hu
阳性样本

Jurkat treated with IFN-α, HeLa treated with IFN-α
预测反应种属
(反应种属缩写表)

Pg, Cw
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu
IP	1:50	Hu
ICC	1:500	Hu
ChIP	1:20-1:50	Hu

背景介绍

Phospho-Stat3 (Tyr705) refers to the phosphorylated form of Signal Transducer and Activator of Transcription 3 (STAT3) at the tyrosine 705 residue. Phosphorylation of STAT3 at Tyr705 is a critical step in the activation of the STAT3 signaling pathway. This phosphorylation event, often induced by cytokines, growth factors, and oncogenes, leads to the formation of STAT3 dimers, which then translocate to the nucleus to initiate gene transcription. STAT3 is involved in various cellular processes, including cell growth, survival, and immune responses. Phospho-Stat3 (Tyr705) is essential for mediating cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors. In the context of immunity, STAT3 plays a crucial role in regulating the differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg), which is controlled by the phosphorylation at Tyr705. Abnormal activation of STAT3, including constitutive phosphorylation at Tyr705, has been observed in various cancers and is associated with tumor growth, survival, and angiogenesis. In some cases, STAT3 activation is driven by autocrine secretion of interleukin-6 (IL-6), leading to continuous activation of the Jak/Stat pathway.

免疫印迹
- WB result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
  Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: untreated Jurkat whole cell lysate 20 µg
  Lane 2: Jurkat treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
  Lane 3: untreated HeLa whole cell lysate 20 µg
  Lane 4: HeLa treated with 50 ng/ml IFN-α for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 88 kDa
  Observed MW: 88 kDa
免疫沉淀
- Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Stat3 (Tyr705) in 0.4 mg Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate 40 µg (Input)
  Lane 2: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb IP in Jurkat+IFN-α (50 ng/ml, 30 min)whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in Jurkat+IFN-α (50 ng/ml, 30 min) whole cell lysate
  Predicted MW: 88 kDa
  Observed MW: 88 kDa
  Exposure time: 90 s
斑点杂交
- Dot blot result of Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb
  Lane1: Stat3 (Tyr705) phospho peptide
  Lane2: Stat3 unmodified peptide
  Primary antibody: Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of HeLa cells treated with IFN-α (50 ng/ml, 30 min)(top panel) and untreated HeLa cells (below panel). Anti- Phospho-Stat3 (Tyr705) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa + IFN-α (50 ng/ml, 30 min) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
  Parallel reactions used Phospho-Stat3 (Tyr705) Recombinant Rabbit mAb (S-515-360) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  
  qPCR showed the enrichment of c-FOS, IRF1 and SAT-α in Phospho-Stat3 (Tyr705)
  Recombinant Rabbit mAb (S-515-360)-immunoprecipitated sample.