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Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb (S-1254-13)

Tyrosine-protein phosphatase non-receptor type 11,Protein-tyrosine phosphatase 1D (PTP-1D),Protein-tyrosine phosphatase 2C (PTP-2C),SH-PTP2 (SHP-2,Shp2),SH-PTP3,PTPN11,PTP2C,SHPTP2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1093

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-SHP-2 (Tyr542)
分子别名

Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D (PTP-1D); Protein-tyrosine phosphatase 2C (PTP-2C); SH-PTP2 (SHP-2; Shp2); SH-PTP3; PTPN11; PTP2C; SHPTP2
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

Q06124
克隆号

S-1254-13
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
阳性样本

NIH/3T3 treated with PDGF, C6
预测反应种属
(反应种属缩写表)

Ck
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB

IP
稀释度

应用稀释度推荐种属

WB 1:5000 Ms, Rt
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:5000	Ms, Rt
IP	1:50	Hu

背景介绍

SHP-2, also known as PTPN11, is a non-receptor protein tyrosine phosphatase containing two Src homology 2 (SH2) domains and a phosphatase domain. Phosphorylation at Tyr542 and another site, Tyr580, in the C-terminal tail are crucial for SHP-2 activation and function as binding sites for proteins with SH2 domains, such as GRb2. SHP-2 is a key regulator in multiple signaling pathways, including the Ras-Raf-MEK-ERK, JAK-STAT, PI3K-AKT-mTOR, and PD-1/PD-L1 pathways. It can act as both an oncogene and a tumor suppressor, depending on the cellular context. SHP-2 plays a role in various cellular processes such as cell growth, differentiation, mitotic cycle, and oncogenic transformation. It is widely expressed in most tissues and is involved in signal transduction from the cell surface to the nucleus. SHP-2 is involved in regulating immune cell functions in the tumor microenvironment and is a target for cancer immunotherapy. It plays a role in the downstream signaling of immune-inhibitory receptors such as PD-1, which is a key target for cancer immunotherapy.

免疫印迹
- WB result of Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb
  Primary antibody: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 starve 18 hours, then treated with 50 ng/ml PDGF for 20 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 68 kDa
- WB result of Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb
  Primary antibody: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 68 kDa
  Observed MW: 68 kDa
免疫沉淀
- Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Stat3 (Tyr705) in 0.4 mg NIH/3T3+starved for 18 hours then +PDGF-treated (50ng/ml, 20 minutes) whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb at 1/50000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: NIH/3T3+starved for 18 hours then +PDGF-treated (50ng/ml, 20 minutes) whole cell lysate 40 µg (Input)
  Lane 2: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb IP in NIH/3T3+starved for 18 hours then +PDGF-treated (50ng/ml, 20 minutes) whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in NIH/3T3+starved for 18 hours then +PDGF-treated (50ng/ml, 20 minutes) whole cell lysate
  Predicted MW: 68 kDa
  Observed MW: 68 kDa