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Rabbit抗原名称
Park7/DJ-1分子别名
Parkinson disease protein 7; Maillard deglycase; Oncogene DJ1; Parkinsonism-associated deglycase; Protein DJ-1; Protein/nucleic acid deglycase DJ-1免疫原
Synthetic Peptide细胞定位
Cell membrane, Cytoplasm, NucleusAccession
Q99497克隆号
S-980-144抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
U-2 OS, Jurkat, MCF7, SK-OV-3, HeLa, NIH/3T3, mouse brain, C6, PC-12, rat brain预测反应种属
(反应种属缩写表)Ck, Bv, Hm, Dr, AfGrMk纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:5000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:500 Hu
Park7 (DJ-1) is a small protein of 189 amino acids that is ubiquitously expressed in almost all cells and tissues, including the brain. It exists as a homodimer and is localized to the cytoplasm, mitochondria, and nucleus. DJ-1 belongs to the ThiJ/PfpI protein superfamily and is highly conserved across all biological kingdoms. Mutations in the Park7 gene, including L166P or deletions, are associated with an early-onset form of familial Parkinson's disease (PD). DJ-1 plays remarkable roles in mitochondrial function and antioxidant effects, and its overexpression can protect cells against dopamine- or MPTP-induced neurotoxicity and oxidative stress. DJ-1 is thought to act as a sensor of cellular redox homeostasis and participate in cytoprotective signaling pathways within the cell. Under oxidative stress, a conserved cysteine residue in DJ-1 (Cys106) is oxidized, which is believed to enable the neuroprotective activity of DJ-1. DJ-1 interacts with various proteins, including RACK1 and MAO-B, to regulate cellular signaling and protect against neurotoxicity. The loss of DJ-1 function can lead to the upregulation of MAO-B, contributing to dopamine metabolic dysfunction and neurodegeneration.
免疫印迹
WB result of Park7/DJ-1 Recombinant Rabbit mAb
Primary antibody: Park7/DJ-1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDaWB result of Park7/DJ-1 Recombinant Rabbit mAb
Primary antibody: Park7/DJ-1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDaWB result of Park7/DJ-1 Recombinant Rabbit mAb
Primary antibody: Park7/DJ-1 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 22 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) labelling Park7/DJ-1 antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Park7/DJ-1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti- Park7/DJ-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Park7/DJ-1 in human tissue
Expression of Park7/DJ-1 in tumor tissue
Expression of Park7/DJ-1 in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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