分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
DARPP32分子别名
Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q9UD71克隆号
S-1637-62抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human brain, mouse brain, rat brain预测反应种属
(反应种属缩写表)Bv, Pg纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:4000 Ms IHC-P 1:100-1:250 Hu, Ms, Rt IF 1:400 Ms
DARPP32, or dopamine- and cAMP-regulated phosphoprotein with an apparent molecular weight of 32 kDa, is a signaling protein that plays a crucial role in the brain, particularly in regions rich in dopaminergic nerve terminals. It is known to be a potent inhibitor of protein phosphatase 1 (PP1) when phosphorylated at Thr34 by cAMP-dependent protein kinase (PKA). DARPP32 is also phosphorylated at other sites by various kinases, which modulates its properties. For instance, phosphorylation at Thr75 by Cdk5 converts DARPP32 into an inhibitor of PKA. This dual functionality makes DARPP32 a key integrator in signal transduction pathways. DARPP-32 is involved in the regulation of a range of physiological and behavioral responses to various stimuli, including neurotransmitters like dopamine and glutamate, as well as drugs of abuse such as cocaine, amphetamine, nicotine, and caffeine. The phosphorylation state of DARPP-32 is intricately regulated, with phosphorylation at Ser102 and Ser137 by CK2 and CK1, respectively, influencing its activity. This complex regulation enables DARPP-32 to modulate the activity of PP-1 and PKA, which in turn affects electrophysiological, transcriptional, and behavioral responses. Moreover, DARPP-32 has been implicated in several psychiatric and neurological disorders, including schizophrenia, and its expression has been observed in a variety of epithelial cells beyond the central nervous system. High levels of DARPP-32 have been associated with survival in certain cancers, suggesting it may play a role in tumorigenesis and could be a potential therapeutic target.
免疫印迹
WB result of DARPP32 Recombinant Rabbit mAb
Primary antibody: DARPP32 Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 32 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-DARPP32 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-DARPP32 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-DARPP32 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-DARPP32 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-DARPP32 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- DARPP32 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of DARPP32 in human tissue
Expression of DARPP32 in tumor tissue
Expression of DARPP32 in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)