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FTO Recombinant Rabbit mAb (S-1663-48)

Alpha-ketoglutarate-dependent dioxygenase FTO,Fat mass and obesity-associated protein,U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO,U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO,mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO (m6A(m)-demethylase FTO),mRNA N(6)-methyladenosine demethylase FTO,tRNA N1-methyl adenine demethylase FTO,KIAA1752

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1085

规格

25μl2ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

FTO
分子别名

Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO (m6A(m)-demethylase FTO); mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO; KIAA1752
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

Q9C0B1
克隆号

S-1663-48
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, SH-SY5Y, 293T, U-87 MG, NIH/3T3, C2C12, PC-12
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:200 Hu, Ms, Rt
ICC 1:500 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
IHC-P	1:200	Hu, Ms, Rt
ICC	1:500	Hu, Ms

背景介绍

FTO (Fat mass and obesity-associated protein) is an enzyme that acts as an RNA N6-methyladenosine (m6A) demethylase. FTO is the first identified demethylase for internal m6A modification in RNA, which is a dynamic and reversible process. It has the capability to remove the methyl group from m6A and N6,2-O-dimethyladenosine (m6Am) in RNA, primarily found in the 5’-UTR. FTO has been reported to be upregulated in many tumors, and its high expression is associated with shorter overall survival in multiple types of cancer patients. It plays an oncogenic role in acute myeloid leukemia (AML) and is involved in cancer stem cell self-renewal and immune evasion. Targeting FTO suppresses cancer stem cell maintenance and immune evasion, suggesting its critical roles in these processes and highlighting the potential of targeting FTO for cancer therapy. FTO is also implicated in epitranscriptomics, which involves chemical modifications in RNA that affect the fate of modified RNAs.

免疫印迹
- WB result of FTO Recombinant Rabbit mAb
  Primary antibody: FTO Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: SH-SY5Y whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Lane 4: U-87 MG whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 58 kDa
- WB result of FTO Recombinant Rabbit mAb
  Primary antibody: FTO Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 58 kDa
- WB result of FTO Recombinant Rabbit mAb
  Primary antibody: FTO Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 58 kDa
免疫沉淀
- FTO Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating FTO in 0.4 mg SH-SY5Y whole cell lysate.
  Western blot was performed on the immunoprecipitate using FTO Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: SH-SY5Y whole cell lysate 20 µg (Input)
  Lane 2: FTO Rabbit mAb IP in SH-SY5Y whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in SH-SY5Y whole cell lysate
  Predicted MW: 58 kDa
  Observed MW: 58 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-FTO antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-FTO antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human clear cell carcinoma of kidney. Anti-FTO antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-FTO antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-FTO antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in SH-SY5Y cells. Anti-FTO antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti-FTO antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of FTO in tumor tissue
- Expression of FTO in human tissue
- Expression of FTO in mouse & rat tissue