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Rabbit抗原名称
Aquaporin 5/AQP5免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9WTY4克隆号
S-1610-43抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
Mouse lung, rat lung纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IP 1:50:00 IHC-P 1:250 Ms, Rt IF 1:500 Ms, Rat
Aquaporin 5 (AQP5) is a member of the aquaporin family of proteins, which are known for their role in the transport of water and small solutes across cell membranes. AQP5 is highly conserved and plays a significant role in various biological functions throughout the human body. AQP5 is crucial for maintaining water homeostasis and has been implicated in several pathological conditions. It is abundantly expressed in different types of tumors, such as those in the respiratory, digestive, and reproductive systems, and may serve as a prognostic biomarker to assess the aggressiveness of cancer. Research has suggested that AQP5 may enhance cancer cell proliferation, migration, and survival in a variety of malignancies.
免疫印迹
WB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDaWB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 23 kDa
免疫沉淀
Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Aquaporin 5/AQP5 in 0.4 mg Mouse lung whole cell lysate.
Western blot was performed on the immunoprecipitate using Aquaporin 5/AQP5 Recombinant Rabbit mAb (S-1245-113) at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Mouse lung whole cell lysate 20 µg (Input)
Lane 2: Aquaporin 5/AQP5 Recombinant Rabbit mAb IP in Mouse lung whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Mouse lung whole cell lysate
Predicted MW: 28 kDa
Observed MW: 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Aquaporin 5/AQP5 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Aquaporin 5/AQP5 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-Aquaporin 5/AQP5 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse lung. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat lung. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Aquaporin 5/AQP5 in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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