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MCM7 Recombinant Rabbit mAb (S-828-221)

DNA replication licensing factor MCM7,CDC47 homolog,P1.1-MCM3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1081
规格
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    MCM7

  • 分子别名

    DNA replication licensing factor MCM7; CDC47 homolog; P1.1-MCM3

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    P33993

  • 克隆号

    S-828-221

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    A431, MCF7, HeLa, Jurkat, NIH/3T3, Neuro-2a, C2C12, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
背景介绍

  • MCM7, or minichromosome maintenance complex component 7, is a highly conserved protein that plays an essential role in the initiation of eukaryotic genome replication. MCM7 is part of the mini-chromosome maintenance proteins (MCM) family, which form a hexameric protein complex known as the pre-replication complex (pre-RC). This complex is crucial for the formation of replication forks and the recruitment of other DNA replication-related proteins. MCM7 has been found to associate with cyclin D1-dependent kinase, CDK4, which may regulate the binding of MCM7 with the tumor suppressor protein RB1/RB, impacting cell cycle progression. MCM7 is often overexpressed in various cancers and is considered a potential proliferation marker. Its high expression is associated with poor prognosis in hepatocellular carcinoma (HCC) and other types of cancer.

  • 免疫印迹

    • WB result of MCM7 Recombinant Rabbit mAb
      Primary antibody: MCM7 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A431 whole cell lysate 20 µg
      Lane 2: MCF7 whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 81 kDa
      Observed MW: 81 kDa

    • WB result of MCM7 Recombinant Rabbit mAb
      Primary antibody: MCM7 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: Neuro-2a whole cell lysate 20 µg
      Lane 3: C2C12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 81 kDa
      Observed MW: 81 kDa

    • WB result of MCM7 Recombinant Rabbit mAb
      Primary antibody: MCM7 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 81 kDa
      Observed MW: 81 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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