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Rabbit抗原名称
CD37分子别名
Leukocyte antigen CD37; Tetraspanin-26 (Tspan-26); TSPAN26免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P11049克隆号
S-1662-61抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Raji, Ramos, Daudi纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
FCM
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu ICC 1:500 Hu FCM 1:500 Hu
CD37 is a cell surface glycoprotein belonging to the tetraspanin superfamily, predominantly expressed on mature B cells and at lower levels in other healthy hematopoietic cells. It interacts with various adhesion molecules, growth and signal transduction receptors, and other tetraspanins, playing a role in membrane organization, signal transduction, survival, and apoptosis. CD37 is expressed in B-cell non-Hodgkin lymphoma (B-NHL), but not all B-cell malignancies express CD37, such as 60% of follicular lymphomas (FL) and 40% of diffuse large B-cell lymphomas (DLBCL). Additionally, CD37 is detected at high levels in T-cell lymphomas and acute myeloid leukemia (AML). Functionally, CD37 plays a role in pro-survival and pro-apoptotic signaling through the PI3K/AKT pathway and controls IL-6 receptor signaling via interaction with SOCS3. It can also mediate the clustering of α4β1 integrin, activating PI3K/AKT signaling and cell survival, but at the same time, it can form complexes with SHP1, LYN, SYK, and PI3Kγ, leading to AKT inactivation and recruitment of SOCS3, limiting the IL6/STAT3 loop.
免疫印迹
WB result of CD37 Recombinant Rabbit mAb
Primary antibody: CD37 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled K562 whole cell lysate 20 µg
Lane 2: unboiled Raji whole cell lysate 20 µg
Lane 3: unboiled Daudi whole cell lysate 20 µg
Lane 4: unboiled Ramos whole cell lysate 20 µg
Negative control: unboiled K562 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 35~55 kDa
流式分析
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) labelling CD37 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: K562
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD37 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD37 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CD37 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CD37 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD37 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adneocarcinoma. Anti-CD37 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Ramos cells (top panel) and negative staining in K562 cells (below panel). Anti-CD37 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CD37 in tumor tissue
Expression of CD37 in human tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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