Keap1 Recombinant Rabbit mAb (S-1520-8)

Kelch-like ECH-associated protein 1,Cytosolic inhibitor of Nrf2 (INrf2),INRF2,KIAA0132,KLHL19

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1078

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Keap1
分子别名

Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2 (INrf2); INRF2; KIAA0132; KLHL19
免疫原

Recombinant Protein
细胞定位

Nucleus, Cytoplasm
Accession

Q14145
克隆号

S-1520-8
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

NK-92, MCF7, HepG2, Jurkat, HeLa, mouse lung, mouse kidney
预测反应种属
(反应种属缩写表)

Gor, Pg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

Keap1 (Kelch-like ECH-associated protein 1) is a critical intracellular sensor and regulator of the Nrf2/ARE (antioxidant response element) signaling pathway. Keap1 is an adaptor protein for the Cul3-based E3 ubiquitin ligase complex, responsible for targeting Nrf2 (nuclear factor erythroid 2-related factor 2) for ubiquitination and proteasomal degradation. It contains 27 cysteine residues that can be modified by oxidative stress, leading to the dissociation of Nrf2 and its subsequent stabilization and nuclear translocation. Under normal conditions, Keap1 binds to Nrf2, targeting it for degradation. However, in response to oxidative stress, certain cysteine residues in Keap1 are modified, inhibiting its ubiquitin ligase activity and leading to Nrf2 accumulation in the nucleus where it activates the transcription of antioxidant and detoxifying genes. Mutations in Keap1 or its interaction with Nrf2 have been implicated in various diseases, including cancer, where somatic mutations in cancer cells occur with high frequency in the DLGex and ETGE motifs of Nrf2, disrupting the Keap1-Nrf2 binding and leading to constitutive accumulation of Nrf2, which supports malignant growth.

免疫印迹
- WB result of Keap1 Recombinant Rabbit mAb
  Primary antibody: Keap1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NK-92 whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: HepG2 whole cell lysate 20 µg
  Lane 4: Jurkat whole cell lysate 20 µg
  Lane 5: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 60 kDa
- WB result of Keap1 Recombinant Rabbit mAb
  Primary antibody: Keap1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse lung lysate 20 µg
  Lane 2: mouse kidney lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 60 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-Keap1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-Keap1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-Keap1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Keap1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Keap1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Keap1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Keap1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti- Keap1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of Keap1 in human tissue
- Expression of Keap1 in tumor tissue
- Expression of Keap1 in mouse & rat tissue