热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/3）
申请试用

Netrin-1 Recombinant Rabbit mAb (S-1510-104)

Epididymis tissue protein Li 131P,NTN1,NTN1L

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1077
规格
数量
收藏 分享

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Netrin-1

  • 分子别名

    Epididymis tissue protein Li 131P; NTN1; NTN1L

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Secreted

  • Accession

    O95631

  • 克隆号

    S-1510-104

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Ms, Rt

  • 阳性样本

    Mouse heart, C6

  • 预测反应种属
    (反应种属缩写表)

    Pg, Ck

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB ,
    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Ms, Rt
    IP 1:50 Ms
背景介绍

  • Netrin-1 is an embryonic, secreted, laminin-related glycoprotein that plays key roles in neuronal navigation, angiogenesis, and cell survival. It is primarily expressed during embryonic development but is also re-expressed by both cancer cells and the tumor microenvironment in a significant proportion of human neoplasms, including inflammation-associated colorectal cancer, metastatic breast cancer, lung cancer, neuroblastoma, lymphoma, and melanoma. Netrin-1 has been shown to stimulate epithelial-mesenchymal transition (EMT) in tumor cells. In the context of cancer, Netrin-1 has been implicated in tumor progression and resistance to chemotherapy. Targeting Netrin-1 with a monoclonal antibody (NP137) that blocks the Netrin-1–UNC5B interaction has shown promise in reducing tumor growth and EMT features in preclinical models and in patients with advanced solid tumors, including endometrial cancer. This targeting strategy not only reduces tumor cell numbers but also increases tumor sensitivity to chemotherapy by inhibiting EMT.

  • 免疫印迹

    • WB result of Netrin-1 Recombinant Rabbit mAb
      Primary antibody: Netrin-1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse heart lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 68 kDa
      Observed MW: 80 kDa

    • WB result of Netrin-1 Recombinant Rabbit mAb
      Primary antibody: Netrin-1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 68 kDa
      Observed MW: 80 kDa

  • 免疫沉淀

    • Netrin-1 Recombinan Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Netrin-1 in 0.4 mg mouse heart whole cell lysate.
      Western blot was performed on the immunoprecipitate using Netrin-1 Recombinan Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: mouse heart whole cell lysate 20 µg (Input)
      Lane 2: Netrin-1 Rabbit mAb IP in mouse heart whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in mouse heart whole cell lysate
      Predicted MW: 68 kDa
      Observed MW: 80 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

评论(0)