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宿主来源
Rat抗原名称
TNFα分子别名
Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2 (TNF-a)细胞定位
SecretedAccession
P06804克隆号
XT3.11抗体类型
Rat mAb抗体同种型
Rat IgG1同型对照
Invivo rat IgG1 Isotype control, anti-trinitrophenol反应种属 ?
Ms纯化方式
Protein G浓度
5 mg/ml纯度
>95% (Determined by SDS-PAGE)内毒素含量
<2EU/mg标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, containing no preservative
储存条件
2 to 8 °C for 2 weeks under sterile conditions;
-20 °C for 3 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
应用
in vitro TNFα neutralization
in vivo TNFα neutralization
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
Tumor Necrosis Factor-alpha (TNF-α) is a homotrimeric protein consisting of 157 amino acids and serves as a potent pro-inflammatory cytokine. It plays a crucial role in various biological functions, including immune responses, inflammatory processes, cell growth, and apoptosis, by binding to its receptors TNFR1 and TNFR2. The dysregulation of TNF-α is associated with a multitude of diseases, such as autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis, ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. In the treatment of autoimmune diseases, TNF-α inhibitors have become an essential therapeutic strategy, with examples including infliximab, etanercept, and adalimumab. In the context of in vitro diagnostic (IVD) applications, TNF-α is often measured in the blood to assess inflammatory responses across a broad spectrum of diseases.
免疫印迹
WB result of Invivo anti-mouse TNFα Recombinant mAb
Primary antibody: Invivo anti-mouse TNFα Recombinant mAb at 1/1000 dilution
Lane 1: TNF-α Protein, Human 10 µg
Lane 2: TNF-α Protein, Mouse 10 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 17 kDa
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