Phospho-Src Family (Tyr416) Recombinant Rabbit mAb (S-1556-29)

Proto-oncogene tyrosine-protein kinase Src,Proto-oncogene c-Src,SRC1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1068

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Src Family (Tyr416)
分子别名

Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; SRC1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P12931
克隆号

S-1556-29
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms
阳性样本

A431 treated with Pervanadate, NIH/3T3 treated with Pervanadate
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ? ,
WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu, Ms
ICC 1:500 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu, Ms
ICC	1:500	Hu

背景介绍

Phospho-Src Family (Tyr416) refers to the Src family of protein tyrosine kinases when phosphorylated at the tyrosine residue 416. This post-translational modification is crucial for the activation of Src kinases, which include Src, Lyn, Fyn, Yes, Lck, Blk, and Hck. Phosphorylation at Tyr416, located within the activation loop of the kinase domain, upregulates enzyme activity, while phosphorylation at another regulatory site, Tyr527, downregulates it. In the context of cancer, Src activity is often dysregulated, contributing to tumorigenesis and cancer progression. Understanding the mechanisms of Src activation, such as phosphorylation at Tyr416, is important for developing targeted therapies against cancer.

免疫印迹
- WB result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
  Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated A431 whole cell lysate 20 µg
  Lane 2: A431 treated with 50 mM Pervanadate for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 60 kDa
- WB result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
  Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with 10 mM Pervanadate for 20 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 60 kDa
斑点杂交
- Dot blot result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
  Lane1: Src famly (Tyr416) phospho peptide
  Lane2: Src famly (Tyr416) unmodified peptide
  Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
- ICC analysis of A431 cells treated with pervanadate (50mM, 5min) (top panel) and A431 cells untreated with pervanadate (50mM, 5min) (below panel). Anti- Phospho-Src Family (Tyr416) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).