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Rabbit抗原名称
Phospho-Src Family (Tyr416)分子别名
Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; SRC1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P12931克隆号
S-1556-29抗体类型
Recombinant mAb抗体同种型
IgG应用
ICC, WB反应种属 ?
Hu, Ms阳性样本
A431 treated with Pervanadate, NIH/3T3 treated with Pervanadate纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Phospho-Src Family (Tyr416) refers to the Src family of protein tyrosine kinases when phosphorylated at the tyrosine residue 416. This post-translational modification is crucial for the activation of Src kinases, which include Src, Lyn, Fyn, Yes, Lck, Blk, and Hck. Phosphorylation at Tyr416, located within the activation loop of the kinase domain, upregulates enzyme activity, while phosphorylation at another regulatory site, Tyr527, downregulates it. In the context of cancer, Src activity is often dysregulated, contributing to tumorigenesis and cancer progression. Understanding the mechanisms of Src activation, such as phosphorylation at Tyr416, is important for developing targeted therapies against cancer.
免疫印迹
WB result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 50 mM Pervanadate for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDaWB result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 10 mM Pervanadate for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
斑点杂交
Dot blot result of Phospho-Src Family (Tyr416) Recombinant Rabbit mAb
Lane1: Src famly (Tyr416) phospho peptide
Lane2: Src famly (Tyr416) unmodified peptide
Primary antibody: Phospho-Src Family (Tyr416) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫细胞化学
ICC analysis of A431 cells treated with pervanadate (50mM, 5min) (top panel) and A431 cells untreated with pervanadate (50mM, 5min) (below panel). Anti- Phospho-Src Family (Tyr416) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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