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Rabbit抗原名称
Parvalbumin分子别名
Parvalbumin alpha, PVALB免疫原
Recombinant Protein细胞定位
CytoplasmAccession
P20472克隆号
S-1620-10抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human cerebellum, mouse skeletal muscle, mouse cerebellum, rat skeletal muscle, rat cerebellum预测反应种属
(反应种属缩写表)Mk, Bv, Rb, Hm, Du, Ct纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
稀释度
应用 稀释度 推荐种属 WB 1:20000 Ms, Rt IHC-P 1:500-1:2000 Hu, Ms, Rt IF 1:100-1:500 Hu, Ms, Rt
Parvalbumin is a small calcium-binding protein that belongs to the EF-hand protein family. It is expressed in vertebrates and is typically found in high concentrations in certain skeletal muscle fibers and neurons. Parvalbumin plays a crucial role as a mobile cytosolic Ca2+ buffer, influencing the duration of intracellular Ca2+ signals and is key in muscle relaxation following contraction and neuronal recovery following excitation. In the context of neuroscience, parvalbumin is highly expressed in the nervous system during development and is associated with the maturation of functional circuits. It is predominantly found in a specific subgroup of cortical interneurons and is involved in the inhibition of neuronal activity. Parvalbumin-expressing neurons are known for their fast-spiking behavior and are involved in various neurological processes, including the regulation of neuronal circuits and the modulation of synaptic transmission.
免疫印迹
WB result of Parvalbumin Recombinant Rabbit mAb
Primary antibody: Parvalbumin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: mouse skeletal muscle lysate 5 µg
Lane 2: mouse cerebellum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 14 kDaWB result of Parvalbumin Recombinant Rabbit mAb
Primary antibody: Parvalbumin Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: rat skeletal muscle lysate 5 µg
Lane 2: rat cerebellum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 14 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human stomach. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-Parvalbumin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebellum. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Parvalbumin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human cerebellum. Anti- Parvalbumin antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebellum. Anti- Parvalbumin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebellum. Anti- Parvalbumin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Parvalbumin in tumor tissue
Expression of Parvalbumin in human tissue
Expression of Parvalbumin in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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