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Mouse抗原名称
CD90分子别名
Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen, THY1细胞定位
Cell membraneAccession
P04216克隆号
5E10抗体类型
Mouse mAb抗体同种型
IgG1反应种属 ?
RhMk, CyMk, Hu阳性样本
U-2 OS, HuT 78, Jurkat纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
FCM
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu FCM 1:2000 Hu
Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
免疫印迹
WB result of Mouse anti-Human CD90 Antibody
Primary antibody: Mouse anti-Human CD90 Antibody at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: HuT 78 whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 23~37 kDa
流式分析
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling Human CD90 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: K562
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD90 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD90 in tumor tissue
Expression of CD90 in human tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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