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IKAROS Recombinant Rabbit mAb (S-729-146)

DNA-binding protein Ikaros,Ikaros family zinc finger protein 1,Lymphoid transcription factor LyF-1,IKZF1,IK1,LYF1,ZNFN1A1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1062

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

IKAROS
分子别名

DNA-binding protein Ikaros; Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1; IKZF1; IK1; LYF1; ZNFN1A1
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q13422
克隆号

S-729-146
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

Raji, Ramos, Molt-4
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IP 1:50 Hu
IHC-P 1:1000 Hu
ICC 1:500 Hu
ICFCM 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IP	1:50	Hu
IHC-P	1:1000	Hu
ICC	1:500	Hu
ICFCM	1:500	Hu

背景介绍

Ikaros is a transcription factor that is encoded by the IKZF genes of the Ikaros family zinc finger group. Zinc finger is a small structural motif of protein that allows protein binding to DNA or RNA molecule that is characterized by the coordination of one or more zinc ions (Zn2+) in order to stabilize the fold. Ikaros displays crucial functions in the hematopoietic system and is a known regulator of immune cells development, mainly in early B cells, CD4+ T cells. Its dysfunction has been linked to the development of chronic lymphocytic leukemia. In particular, Ikaros has been found in recent years to be a major tumor suppressor involved in human B-cell acute lymphoblastic leukemia and that it also has a part in the differentiation and function of individual T helper cells.

免疫印迹
- WB result of IKAROS Recombinant Rabbit mAb
  Primary antibody: IKAROS Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Raji whole cell lysate 20 µg
  Lane 3: Ramos whole cell lysate 20 µg
  Lane 4: Molt-4 whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 58 kDa
  Observed MW: 50~70 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / Ramos (Human Burkitt's lymphoma B lymphocyte, Right) labelling IKAROS antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: HeLa
免疫沉淀
- IKAROS Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IKAROS in 0.4 mg MOLT4 whole cell lysate.
  Western blot was performed on the immunoprecipitate using IKAROS Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: MOLT4 whole cell lysate 40 µg (Input)
  Lane 2: IKAROS Recombinant Rabbit mAb IP in MOLT4 whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in MOLT4 whole cell lysate
  Predicted MW: 58 kDa
  Observed MW: 50~70 kDa
  Exposure time: 180 s
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti- IKAROS antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- IKAROS antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in Ramos cells (top panel) and negative staining in HeLa cells (below panel). Anti- IKAROS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).