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Rabbit抗原名称
IKAROS分子别名
DNA-binding protein Ikaros; Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1; IKZF1; IK1; LYF1; ZNFN1A1免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q13422克隆号
S-729-146抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB, IP反应种属 ?
Hu阳性样本
Raji, Ramos, Molt-4纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Ikaros is a transcription factor that is encoded by the IKZF genes of the Ikaros family zinc finger group. Zinc finger is a small structural motif of protein that allows protein binding to DNA or RNA molecule that is characterized by the coordination of one or more zinc ions (Zn2+) in order to stabilize the fold. Ikaros displays crucial functions in the hematopoietic system and is a known regulator of immune cells development, mainly in early B cells, CD4+ T cells. Its dysfunction has been linked to the development of chronic lymphocytic leukemia. In particular, Ikaros has been found in recent years to be a major tumor suppressor involved in human B-cell acute lymphoblastic leukemia and that it also has a part in the differentiation and function of individual T helper cells.
免疫印迹
WB result of IKAROS Recombinant Rabbit mAb
Primary antibody: IKAROS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 50~70 kDa
免疫沉淀
IKAROS Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IKAROS in 0.4 mg MOLT4 whole cell lysate.
Western blot was performed on the immunoprecipitate using IKAROS Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: MOLT4 whole cell lysate 40 µg (Input)
Lane 2: IKAROS Recombinant Rabbit mAb IP in MOLT4 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in MOLT4 whole cell lysate
Predicted MW: 58 kDa
Observed MW: 50~70 kDa
Exposure time: 180 s
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti- IKAROS antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- IKAROS antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Ramos cells (top panel) and negative staining in HeLa cells (below panel). Anti- IKAROS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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