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Rabbit抗原名称
U2AF2分子别名
Splicing factor U2AF 65 kDa subunit; U2 auxiliary factor 65 kDa subunit (hU2AF(65); hU2AF65); U2 snRNP auxiliary factor large subunit; U2AF65免疫原
Synthetic Peptide细胞定位
NucleusAccession
P26368克隆号
S-1324-66抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P ? ,ICC ? ,WB ,IP ,IF ?稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu IF 1:500 Hu 反应种属 ?
Hu, Ms, Rt阳性样本
Jurkat , A431, HeLa, HepG2, MCF7, NIH/3T3, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
U2AF2, also known as U2 small nuclear RNA auxiliary factor 2 or U2AF65, is a protein that plays a pivotal role in pre-mRNA splicing. This process is essential for the regulation of gene expression, as it allows a single gene to give rise to multiple protein isoforms through alternative splicing. U2AF2 is part of the U2AF heterodimer, which also includes U2AF1 (U2AF35), and it is responsible for recognizing the 3' splice site during the splicing of pre-mRNA. The protein U2AF2 has a dynamic structure that can switch between open and closed conformations, which affects its ability to bind to the mRNA precursor. This structural flexibility is crucial for the regulation of splicing efficiency at different splice sites. U2AF2 is expressed ubiquitously in the nucleus and is localized to the nucleoplasm and nuclear speckles. It is also detected in the brain, with soma and nucleus in neurons. The protein is involved in several biological processes, including mRNA processing and splicing. U2AF2 is also known to regulate the inclusion of specific exons, such as cardiac troponin-T (TNNT2) premRNA exon inclusion in muscle tissue.
免疫印迹
WB result of U2AF2 Recombinant Rabbit mAb
Primary antibody: U2AF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDaWB result of U2AF2 Recombinant Rabbit mAb
Primary antibody: U2AF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDaWB result of U2AF2 Recombinant Rabbit mAb
Primary antibody: U2AF2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDa
免疫沉淀
U2AF2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating U2AF2 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using U2AF2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: U2AF2 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 53 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- U2AF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti- U2AF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- U2AF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- U2AF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- U2AF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- U2AF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- U2AF2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- U2AF2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded human colon cancer. Anti- U2AF2 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) (S0B4007) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung squamous carcinoma. Anti- U2AF2 antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) (S0B4007) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of U2AF2 in tumor tissue
Expression of U2AF2 in human tissue
Expression of U2AF2 in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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