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SMARCA2/BRM Recombinant Rabbit mAb (S-1568-17)

Probable global transcription activator SNF2L2,ATP-dependent helicase SMARCA2,BRG1-associated factor 190B (BAF190B),Protein brahma homolog (hBRM),SNF2-alpha,SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2,BAF190B,SNF2A,SNF2L2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1059

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

SMARCA2/BRM
分子别名

Probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BRG1-associated factor 190B (BAF190B); Protein brahma homolog (hBRM); SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; BAF190B; SNF2A; SNF2L2
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P51531
克隆号

S-1568-17
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, 293T, HeLa, DU 145, Neuro-2a, mouse brain, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:100 Hu, Ms, Rt
ICC 1:500 Hu, Ms, Rt
ICFCM 1:50

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:100	Hu, Ms, Rt
ICC	1:500	Hu, Ms, Rt
ICFCM	1:50

背景介绍

SMARCA2, also known as BRM (Brahma-related gene on chromosome X), is a subfamily member of the SWI/SNF family of proteins. It plays a crucial role in chromatin remodeling, which involves the adjustment of chromatin structure to facilitate or hinder the access of the transcriptional machinery to DNA. This process is essential for various cellular functions, including gene expression, DNA repair, and replication. As a part of the SWI/SNF complex, SMARCA2 uses the energy from ATP hydrolysis to reposition and evict nucleosomes, thereby altering chromatin structure. This function is vital for transcriptional regulation and maintaining genomic integrity. Mutations or alterations in the expression of SMARCA2 have been implicated in various human diseases, particularly in the context of cancer. It has been found that SMARCA2 mutations can lead to different neurodevelopmental disorders, including Nicolaides-Baraitser syndrome (NCBRS) and Lennox-Gastaut syndrome (LGS). These conditions are often characterized by developmental delays, intellectual disabilities, and epilepsy.

免疫印迹
- WB result of SMARCA2/BRM Recombinant Rabbit mAb
  Primary antibody: SMARCA2/BRM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: DU 145 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 181 kDa
  Observed MW: 220 kDa
- WB result of SMARCA2/BRM Recombinant Rabbit mAb
  Primary antibody: SMARCA2/BRM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Neuro-2a whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 181 kDa
  Observed MW: 220 kDa
- WB result of SMARCA2/BRM Recombinant Rabbit mAb
  Primary antibody: SMARCA2/BRM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 181 kDa
  Observed MW: 230 kDa
  This blot was developed with high sensitivity substrate
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SMARCA2/BRM antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti- SMARCA2/BRM antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- SMARCA2/BRM antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells (top panel) and negative staining in NCCIT cells (below panel). Anti-SMARCA2/BRM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of SMARCA2/BRM in human tissue
- Expression of SMARCA2/BRM in mouse & rat tissue
- Expression of SMARCA2/BRM in tumor tissue