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宿主来源
Rat分子别名
THY1, T25, Thy-1, Thy-1.2细胞定位
Cell membraneAccession
P01831克隆号
53-2.1抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
mouse brain, mouse thymus, C57BL/6 mouse splenocytes纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
FCM
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms FCM 1:200 Ms
CD90.2 protein, also known as Thy-1.2, is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein with a V-type immunoglobulin domain. It is one of the two murine isoforms of the Thy-1 protein, distinguished mainly by the 89th amino acid, where Thy-1.1 carries an arginine and Thy-1.2 carries a glutamine. CD90.2 is expressed in various cell types, including T cells and fibroblasts of certain mouse strains, and plays a role in cell-to-cell and cell-matrix interactions. It is involved in axonal growth and neuronal regeneration, T cell activation and apoptosis, adhesion and migration of leukocytes and melanoma cells, as well as proliferation and migration of fibroblasts in wound healing, inflammation, and fibrosis.
免疫印迹
WB result of Rat anti-Mouse CD90.2 Antibody
Primary antibody: Rat anti-Mouse CD90.2 Antibody at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 22 kDa
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD90.2 antibody at 1/200 (1 μg) dilution (Right) compared with a Rat monoclonal IgG isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ separately. Gated on total viable cells.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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