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Rabbit抗原名称
CD3ε分子别名
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P22646克隆号
S-300-125抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Ms阳性样本
C2C12, EL4.IL2, mouse spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Ms |
| IHC-P | 1:1000 | Ms |
CD3ε, also known as the epsilon chain of the CD3 complex, is a crucial component of the T cell receptor (TCR) complex, which plays a central role in T cell activation and antigen recognition. CD3ε is particularly important in the context of T cell-mediated immune responses. It has been identified as a key target in the development of immunotherapies for cancer. Recent research has also highlighted the role of CD3ε in CAR-T cell therapy. By incorporating the CD3ε cytoplasmic domain into a chimeric antigen receptor (CAR), the antitumor activity of CAR-T cells can be improved.
免疫印迹
WB result of CD3ε Recombinant Rabbit mAb
Primary antibody: CD3ε Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: EL4.IL2 whole cell lysate 20 µg
Negative control: C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 21 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD3ε in mouse & rat tissue
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