CD3ε Recombinant Rabbit mAb (S-300-125)

T-cell surface glycoprotein CD3 epsilon chain,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1056

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CD3ε
分子别名

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e
免疫原

Synthetic Peptide
细胞定位

Cell membrane
Accession

P22646
克隆号

S-300-125
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms
阳性样本

C2C12, EL4.IL2, mouse spleen
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Ms
IHC-P 1:1000 Ms

应用	稀释度	推荐种属
WB	1:1000	Ms
IHC-P	1:1000	Ms

背景介绍

CD3ε, also known as the epsilon chain of the CD3 complex, is a crucial component of the T cell receptor (TCR) complex, which plays a central role in T cell activation and antigen recognition. CD3ε is particularly important in the context of T cell-mediated immune responses. It has been identified as a key target in the development of immunotherapies for cancer. Recent research has also highlighted the role of CD3ε in CAR-T cell therapy. By incorporating the CD3ε cytoplasmic domain into a chimeric antigen receptor (CAR), the antitumor activity of CAR-T cells can be improved.

免疫印迹
- WB result of CD3ε Recombinant Rabbit mAb
  Primary antibody: CD3ε Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Lane 2: EL4.IL2 whole cell lysate 20 µg
  Negative control: C2C12 whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 21 kDa
  Observed MW: 21 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of CD3ε in mouse & rat tissue