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Rabbit抗原名称
GSK3β分子别名
Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B免疫原
Synthetic Peptide细胞定位
Nucleus, Cell membrane, CytoplasmAccession
P49841克隆号
S-1209-102抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, A431, MCF7, NIH/3T3, PC-12预测反应种属
(反应种属缩写表)S. citellus纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt
GSK3β, or glycogen synthase kinase-3 beta, is a serine/threonine protein kinase that plays a crucial role in a variety of cellular processes. It is involved in the molecular pathophysiology of many diseases, including cancer, neurodegenerative disorders, and diabetes. GSK3β is known to regulate mitochondrial activity, which is essential for producing cellular energy and determining cell fates. It has been implicated in the mediation of mitochondrial functions such as biogenesis, bioenergetics, permeability, motility, and apoptosis. In the context of cancer, GSK3β has a dual role. It can act as a tumor suppressor by inhibiting the Wnt/β-catenin signaling pathway, which is often upregulated in various cancers. However, it can also promote tumorigenesis under certain conditions, such as when it phosphorylates and stabilizes β-catenin, leading to uncontrolled cell proliferation. GSK3β is involved in cell survival, proliferation, invasion, and therapy resistance in cancer cells. It also plays a role in the self-renewal and maintenance of cancer stem cells, which are responsible for tumor recurrence and metastasis. GSK3β is also a key player in the regulation of the cell cycle and apoptosis. It phosphorylates various proteins involved in these processes, such as cyclin D1, MYC, BAX, and NF-κB. Inhibition of GSK3β has been shown to induce apoptosis and mitotic catastrophe in cancer cells by disrupting centrosome regulation.
免疫印迹
WB result of GSK3β Recombinant Rabbit mAb
Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46 kDaWB result of GSK3β Recombinant Rabbit mAb
Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46 kDaWB result of GSK3β Recombinant Rabbit mAb
Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of GSK3β in tumor tissue
Expression of GSK3β in human tissue
Expression of GSK3β in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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