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Rabbit抗原名称
ATP5A分子别名
ATP synthase subunit alpha, mitochondrial; ATP synthase F1 subunit alpha; ATP5F1A; ATP5A1; ATP5AL2; ATPM免疫原
Synthetic Peptide细胞定位
Cell membrane, MitochondrionAccession
P25705抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, Jurkat, A549, Neuro-2a, PC-12预测反应种属
(反应种属缩写表)Or, Pg, Bv, Fr, Ys, Nm纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu
ATP5A is the alpha subunit of the mitochondrial ATP synthase. The ATP synthase has two main functional domains: the catalytic core known as F1 and the membrane-embedded proton channel called Fo. The F1 part consists of three alpha subunits (ATP5A1) and three beta subunits (ATP5B), forming a hexamer with six ADP/ATP binding sites. Only the beta subunits are catalytically active in ATP synthesis/hydrolysis, while the alpha subunits are catalytically inactive. ATP5A1 is expressed in various tissues, including the heart and kidneys, and has broad species reactivity, such as in mice, rats, cows, humans, and fruit flies. Beyond its role in energy metabolism, studies suggest that ATP5A1 may also be involved in the post-transcriptional regulation of cancer-related genes and is associated with the development of various diseases.
免疫印迹
WB result of ATP5A Rabbit pAb
Primary antibody: ATP5A Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 50 kDaWB result of ATP5A Rabbit pAb
Primary antibody: ATP5A Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 50 kDaWB result of ATP5A Rabbit pAb
Primary antibody: ATP5A Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 50 kDa
免疫沉淀
ATP5A Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating ATP5A in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using ATP5A Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: ATP5A Rabbit pAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 60 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ATP5A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-ATP5A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in MCF7 cells. Anti-ATP5A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ATP5A in tumor tissue
Expression of ATP5A in human tissue
Expression of ATP5A in mouse & rat tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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