CD123 Recombinant Rabbit mAb (S-1592-12)

Interleukin-3 receptor subunit alpha,IL-3 receptor subunit alpha,IL-3R subunit alpha,IL-3R-alpha,IL-3RA,IL3RA,IL3R

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1050

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CD123
分子别名

Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; IL3RA; IL3R
免疫原

Recombinant Protein
细胞定位

Cell membrane
Accession

P26951
克隆号

S-1592-12
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

THP-1
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IHC-P 1:50-1:100 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IHC-P	1:50-1:100	Hu

背景介绍

CD123, also known as the interleukin-3 receptor alpha chain (IL-3Rα), is a glycoprotein composed of 360 amino acids. It forms a high-affinity IL-3 receptor with CD131, and by binding to the IL-3 ligand, it promotes the proliferation and survival of hematopoietic stem/progenitor cells (HSPCs). In various hematological malignancies, such as acute myeloid leukemia (AML), blastic plasmacytoid dendritic cell neoplasm (BPDCN), acute lymphoblastic leukemia/lymphoma (ALL), hairy cell leukemia (HCL), and systemic mastocytosis (SM), the expression level of CD123 is often high and associated with the overproliferation of tumor cells. It is worth noting that the expression level of CD123 increases with the progression and worsening of hematological malignancies, indicating that the expression level of CD123 may be related to the severity of the disease. Moreover, high expression of CD123 is associated with poor prognosis in AML, making it an ideal target for AML immunotherapy.

免疫印迹
- WB result of CD123 Recombinant Rabbit mAb
  Primary antibody: CD123 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Raji whole cell lysate 20 µg
  Lane 2: THP-1 whole cell lysate 20 µg
  Negative control: Raji whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 75 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD123 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human spleen. Anti-CD123 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenomcarcinoma. Anti-CD123 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of CD123 in tumor tissue
- Expression of CD123 in human tissue