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Rabbit抗原名称
AiolosI/KZF3分子别名
Zinc finger protein Aiolos; Ikaros family zinc finger protein 3; ZNFN1A3免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q9UKT9克隆号
S-1268-42抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Raji, Ramos, Daudi纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu ChIP 1:20-1:50 Hu
Aiolos protein is a transcription factor that belongs to the Ikaros family of zinc finger proteins, encoded by the Ikzf3 gene. It plays a crucial role in the development and function of various immune cells, regulating gene expression directly by promoting the transcription of specific genes and indirectly by silencing gene expression through chromatin remodeling. Aiolos protein is particularly important in the context of tissue-resident type 2 innate immune cells. Studies have shown that Aiolos can shape the transcriptome profile and chromatin landscape, which is essential for the positioning and function of tissue-resident eosinophils and group 2 innate lymphoid cells (ILC2s). Aiolos is specifically expressed in intestinal ILC2s of mice and human colon ILC2s and is associated with tissue residency in the gut. The expression of Aiolos protein in eosinophils and ILC2s is crucial for their tissue positioning and functionality. For instance, Aiolos regulates the expression of CCR3 in eosinophils and the expression of 371 genes associated with ERK/MAPK signaling and chemotaxis. Additionally, Aiolos plays a role in the positioning of eosinophils in the gut and lungs. Furthermore, the expression of Aiolos protein in lung cancer is associated with enhanced proliferation, migration, and invasion capabilities of lung cancer cells. Studies suggest that OTUB1 interacts with Aiolos protein, leading to reduced ubiquitination and increased protein stability of Aiolos, potentially affecting biological functions related to Aiolos, including cell migration and invasion.
免疫印迹
WB result of Aiolos/IKZF3 Recombinant Rabbit mAb
Primary antibody: Aiolos/IKZF3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-937 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Lane 4: Daudi whole cell lysate 20 µg
Negative control: U-937 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 45, 55, 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-AiolosI/KZF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-AiolosI/KZF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-AiolosI/KZF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-AiolosI/KZF3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on Ramos cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used AiolosI/KZF3 Recombinant Rabbit mAb (S-1268-42) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of Hsp90-β, SirT1 and SAT-α in AiolosI/KZF3 Recombinant Rabbit mAb (S-1268-42)-immunoprecipitated sample.
组织表达图谱
Expression of AiolosI/KZF3 in tumor tissue
Expression of AiolosI/KZF3 in human tissue
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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