elF4A1 Rabbit Polyclonal Antibody

Eukaryotic initiation factor 4A-I,eIF-4A-I,eIF4A-I,ATP-dependent RNA helicase eIF4A-1,DDX2A,EIF4A

Datasheet COA

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B1046

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

elF4A1
分子别名

Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1; DDX2A; EIF4A
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P60842
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, HeLa, Jurkat, A431, NIH/3T3, mouse liver, PC-12, C6
预测反应种属
(反应种属缩写表)

CyMk, Or, Rb
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu

背景介绍

eIF4A1, or eukaryotic translation initiation factor 4A1, is a protein that plays a crucial role in the initiation of translation in eukaryotic cells. It is one of the subunits of the eIF4F complex, which is responsible for unwinding the secondary structure of the mRNA 5' untranslated region (5' UTR) to facilitate the binding of the 40S ribosomal subunit to the start codon. Deregulated translation initiation, including the overactivity of eIF4A1, is implicated in cancer initiation and progression. This makes eIF4A1 an attractive target for cancer therapeutics. Researchers are actively pursuing strategies that target eIF4A1 to inhibit tumor growth and proliferation. Several natural product molecules, such as Rocaglamide A (RocA), have been reported to target eIF4A1, but their clinical utilization has been limited due to pharmacological limitations. In the context of cancer, eIF4A1 has been associated with poor outcomes in diffuse large B cell lymphoma (DLBCL). Higher expression levels of eIF4A1 are linked to shorter overall survival and progression-free survival in patients with DLBCL. This suggests that eIF4A1 expression could serve as a prognostic biomarker for this type of cancer. eIF4A1 is also involved in the translation of specific mRNAs that have complex secondary structures or are under translational control by factors such as the mTOR pathway. During mTORC1 inhibition, eIF4A1 enhances the translational repression mediated by LARP1 (La-related protein 1), which is a key regulator of mRNA translation.

免疫印迹
- WB result of elF4A1 Rabbit pAb
  Primary antibody: elF4A1 Rabbit pAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: A431 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 46 kDa
  Observed MW: 46 kDa
- WB result of elF4A1 Rabbit pAb
  Primary antibody: elF4A1 Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 46 kDa
  Observed MW: 46 kDa
- WB result of elF4A1 Rabbit pAb
  Primary antibody: elF4A1 Rabbit pAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 46 kDa
  Observed MW: 46 kDa
免疫沉淀
- elF4A1 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating elF4A1 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using elF4A1 Rabbit pAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: elF4A1 Rabbit pAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 46 kDa
  Observed MW: 46 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- elF4A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).