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Rabbit抗原名称
Calreticulin分子别名
CRP55; Calregulin; Endoplasmic reticulum resident protein 60 (ERp60); HACBP; grp60; CALR; CRTC免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulum, CytoplasmAccession
P27797克隆号
S-779-273抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu预测反应种属
(反应种属缩写表)JaMq纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000 Hu
Calreticulin (CRT) is a multifunctional protein found in the endoplasmic reticulum (ER) of cells. It was initially identified as a calcium-binding protein in skeletal muscle sarcoplasmic reticulum, but it is now known to be abundant in non-muscle tissues as well and plays a variety of roles in cellular processes. As a chaperone, calreticulin helps in the folding of proteins and prevents the premature export of misfolded proteins from the ER. It works in conjunction with other chaperones like calnexin and ERp57. And it buffers calcium ions, which are essential for various cellular signaling pathways. Calreticulin also plays a role in integrin-mediated cell adhesion and calcium signaling. On the surface of cells undergoing immunogenic cell death (ICD), calreticulin can expose and enhance the uptake of cell corpses by phagocytes, thereby supporting anticancer immunity. Moreover, it is part of the peptide-loading complex, which ensures the proper loading of cellular antigens onto MHC Class I molecules. Calreticulin has been implicated in the enhancement of wound healing through multiple biological effects, including the modulation of extracellular matrix components and the promotion of cell proliferation. It also interacts with various components of the immune system, such as C1q and mannan-binding protein, which suggests its involvement in the clearance of apoptotic cells and inflammation resolution.
免疫印迹
WB result of Calreticulin Recombinant Rabbit mAb
Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Calreticulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Calreticulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Calreticulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Calreticulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adeoncarcinoma. Anti-Calreticulin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Calreticulin in tumor tissue.
Expression of human in tumor tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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