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ATRIP Recombinant Rabbit mAb (S-1275-48)

ATR-interacting protein,ATM and Rad3-related-interacting protein,AGS1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1041
规格
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ATRIP

  • 分子别名

    ATR-interacting protein; ATM and Rad3-related-interacting protein; AGS1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q8WXE1

  • 克隆号

    S-1275-48

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu

  • 阳性样本

    PC-3, HeLa, MCF7, HepG2, LNCaP, OVCAR-3, HEK-293

  • 预测反应种属
    (反应种属缩写表)

    Mk, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB ,
    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
    IP 1:50 Hu
背景介绍

  • ATRIP stands for ATR-interacting protein. It works together with ATR (ataxia-telangiectasia mutated and Rad3-related) as a crucial component in the cell cycle checkpoint signaling pathway. Both ATR and ATRIP localize to intranuclear foci following DNA damage or replication inhibition. They are essential for the viability of human somatic cells and are interdependent in the cell cycle checkpoint signaling pathways. Furthermore, ATRIP plays a significant role in the DNA damage response. Together with ATR, it phosphorylates and transmits damage signals to effectors, initiating the repair process.

  • 免疫印迹

    • WB result of ATRIP Recombinant Rabbit mAb
      Primary antibody: ATRIP Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-3 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Lane 5: LNCaP whole cell lysate 20 µg
      Lane 6: OVCAR-3 whole cell lysate 20 µg
      Lane 7: HEK-293 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 86 kDa
      Observed MW: 90 kDa

  • 免疫沉淀

    • ATRIP Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ATRIP in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using ATRIP Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: ATRIP Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 86 kDa
      Observed MW: 90 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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