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ATRIP Recombinant Rabbit mAb (S-1275-48)

ATR-interacting protein,ATM and Rad3-related-interacting protein,AGS1

货号： S0B1041

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ATRIP

  • 分子别名

    ATR-interacting protein; ATM and Rad3-related-interacting protein; AGS1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q8WXE1

  • 克隆号

    S-1275-48

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    WB, IP

  • 反应种属 ?

    Hu

  • 阳性样本

    PC-3, HeLa, MCF7, HepG2, LNCaP, OVCAR-3, HEK-293

  • 预测反应种属
    (反应种属缩写表)

    Mk, Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu
IP 1:50 Hu
背景介绍

  • ATRIP stands for ATR-interacting protein. It works together with ATR (ataxia-telangiectasia mutated and Rad3-related) as a crucial component in the cell cycle checkpoint signaling pathway. Both ATR and ATRIP localize to intranuclear foci following DNA damage or replication inhibition. They are essential for the viability of human somatic cells and are interdependent in the cell cycle checkpoint signaling pathways. Furthermore, ATRIP plays a significant role in the DNA damage response. Together with ATR, it phosphorylates and transmits damage signals to effectors, initiating the repair process.

  • 免疫印迹

    • WB result of ATRIP Recombinant Rabbit mAb
      Primary antibody: ATRIP Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-3 whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Lane 3: MCF7 whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Lane 5: LNCaP whole cell lysate 20 µg
      Lane 6: OVCAR-3 whole cell lysate 20 µg
      Lane 7: HEK-293 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 86 kDa
      Observed MW: 90 kDa

  • 免疫沉淀

    • ATRIP Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ATRIP in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using ATRIP Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: ATRIP Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 86 kDa
      Observed MW: 90 kDa

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