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ROCK1 Recombinant Rabbit mAb (S-1304-153)

Rho-associated protein kinase 1,Renal carcinoma antigen NY-REN-35,Rho-associated,coiled-coil-containing protein kinase 1,ROCK-I,p160 ROCK-1 (p160ROCK)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1040

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

ROCK1
分子别名

Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated, coiled-coil-containing protein kinase 1; ROCK-I; p160 ROCK-1 (p160ROCK)
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q13464
克隆号

S-1304-153
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, HepG2, Jurkat, NIH/3T3, C6
预测反应种属
(反应种属缩写表)

Rb
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ? ,
WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu, Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu, Ms

背景介绍

ROCK1 (Rho-associated coiled-coil kinase 1) is a serine/threonine protein kinase that plays a crucial role in various cellular processes, including cell proliferation, migration, adhesion, and cytoskeletal remodeling. ROCK1 is involved in the regulation of the actin cytoskeleton and stress fiber formation. It achieves this by phosphorylating and modulating the activity of a range of actin-binding proteins and intermediate filament proteins. The kinase activity of ROCK1 is regulated by its interaction with the small GTPase RhoA. When RhoA is activated, it binds to the Rho-binding domain (RBD) of ROCK1, leading to a conformational change that relieves the auto-inhibition of the kinase domain and enhances ROCK1's catalytic activity. ROCK1 has been implicated in the pathogenesis of several diseases, including cancer, cardiovascular diseases, and neurological disorders. In cancer, ROCK1 has been shown to promote cell proliferation and survival, making it a potential therapeutic target for cancer treatment. In the context of cardiovascular diseases, ROCK1 activity has been associated with hypertension and pulmonary hypertension, as well as the regulation of vascular tone and remodeling.

免疫印迹
- WB result of ROCK1 Recombinant Rabbit mAb
  Primary antibody: ROCK1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
- WB result of ROCK1 Recombinant Rabbit mAb
  Primary antibody: ROCK1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
- WB result of ROCK1 Recombinant Rabbit mAb
  Primary antibody: ROCK1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
免疫沉淀
- ROCK1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ROCK1 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using ROCK1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: ROCK1 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 158 kDa
  Observed MW: 160 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- ROCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in NIH/3T3 cells. Anti- ROCK1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).